Goat polyclonal anti actin c 11

The antibodies used in this study were as follows: mouse monoclonal anti-Cx45 (MAB3100, Merck Millipore, Billerica, MA, USA), mouse monoclonal anti-Cx43 (C13720, BD Transduction Laboratories™, San Joes, CA, USA), goat polyclonal anti-actin (C-11, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-mouse IgG (H + L) HRP-conjugated (62-6520, Thermo Fisher Scientific, Rockford, IL, USA) antibodies. RNeasy^® Mini Kit (74104, Qiagen, Hilden, Germany), PrimeScript TM 1st strand cDNA kit (6110A, Takara Bio, Shiga, Japan), Taq polymerase PCR kit (DT95-E500, Solgent, Daejeon, Korea), tissue DNA Purification Kit (CME0112, Cosmogenetech, Daegu, Korea), T7 endonuclease 1 (T7E1) (M0302L, New England Biolabs, Ipswich, MA, USA), and LY (L0144, Sigma-Aldrich, St Louis, MO, USA) were purchased from local vendors.

Mouse monoclonal anti-α-Tubulin, goat polyclonal anti-actin (C-11) and rabbit polyclonal anti-CD40 (N-16) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal anti-caspase-3 and anti-RIP1 antibodies were obtained from Cell Signaling Technology. Mouse monoclonal agonistic anti-CD40 (Clone # 82111) antibody was purchased from R&D Systems (Minneapolis, MN, USA). Horseradish peroxidase-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were obtained from Bio-Rad Laboratories (Hercules, CA, USA). Recombinant human sCD40 ligand (CD40L) was obtained from Peprotech (Rocky Hill, NJ, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), necrostatin-1 and 1-MT were purchased from Sigma-Aldrich. Boc-D-FMK was purchased from Abcam (Toronto, ON, Canada). GSK'872 and necrosulfonamide were purchased from Millipore (Etobicoke, ON, Canada).