Simple stain dab solution

Paraffin-embedded of liver tissues were cut into 5 μm-thick sections, followed by being dewaxed and rehydrated. After washing in 1× phosphate-buffered saline (PBS), nonspecific antigens were blocked by incubation of the slides with 1% normal goat serum for 20 min. The slides were then incubated with 1 : 200 diluted anti-Gr-1 antibody (R&D, USA) at 4 °C for overnight. Slides were treated with anti-rabbit IgG (Servicebio, China) for 30 min and then incubated with simple stain DAB solution (Servicebio, China). Finally, slides were counterstained with hematoxylin, dehydrated, and mounted.
The slides were observed and photographed by a light microscope (Zeiss, Germany).

Paraffin-embedded tumor tissues were sectioned at 5 μm thickness, followed by being dewaxed and rehydrated. After washing in phosphate-buffered saline (PBS), nonspecific antigens were prevented by incubation of the slides with 1% normal goat serum for 20 min. Then the slides were incubated with 1:200 diluted anti-CD161 antibody (ab197979, Abcam, USA) at 4 °C for overnight. Slides were treated with secondary antibody anti-rabbit IgG (Servicebio, China) for 30 min and then incubated with simple stain DAB solution (Servicebio, China). Finally, slides were counterstained with hematoxylin, dehydrated, and mounted.
The slides were observed and photographed by a light microscope using 1×100 and 200 magnification (Zeiss, Germany).