E selectin

TDD was synthesized in our laboratory as previously reported (Supplementary Material online, Figure S1, 99% purity).14 LPS was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Antibodies for phosphor (p)‐ERK1/2, ERK1/2, p‐p38, p38, p‐JNK, JNK, ICAM‐1, VCAM‐1, E‐selectin and NF‐κB/p65 were purchased from Cell Signaling Technology (Beverly, MA, USA). TRIzol reagent, a PrimeScript™ RT reagent kit and an SYBR Premix Ex Taq™ II kit were obtained from TaKaRa Bio, Inc. (Shiga, Japan). Lamin B Rabbit Monoclonal Antibody, a nuclear protein extraction kit, and an NF‐κB Activation‐Nuclear Translocation Assay Kit were obtained from Biotechnology Co. (Shanghai, China). The antibody for HMBOX1 was obtained from Proteintech Group Inc. (Wuhan, China). ELISA kits for IL‐6 and TNF‐α, β‐actin antibody, horseradish peroxidase‐conjugated secondary antibodies and enhanced chemiluminescence (ECL) detection kit were obtained from Boster Biotech Co., Ltd. (Wuhan, China).

RPMI 1640, fetal bovine serum (FBS), phosphate-buffered saline (PBS), TRIzol, SuperScript reverse transcriptase enzyme, and buffer were purchased from Gibco-BRL Company (USA). Dimethyl sulfoxide (DMSO), DEPC, and propidium iodide (PI) were purchased from Sigma-Aldrich Chemical Company (USA). CCK-8 Kit was purchased from DOJINDO Company (Japan). Ethanol, petroleum ether, ethyl acetate, n-butanol, and all other chemicals were of analytical grade. Target gene primers and probes were synthesized from Takar Company. Taq DNA polymerase and buffer were purchased from Roche Company. The antibodies for detecting E-cadherin, E-selectin, CCR7, SLC, SDF-1, and CXCR4 were purchased from Cell Signaling Technology.