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Cd3 cy7pe

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CD3-Cy7PE is a fluorescently labeled antibody that binds to the CD3 antigen, which is expressed on the surface of T cells. It is used as a tool for the identification and analysis of T cells in flow cytometry applications.

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Lab products found in correlation

Igepal, by Thermo Fisher Scientific (2 mentions) Cd20 cy7apc, by BD (2 mentions) Cd3 cd20 cd19, by Thermo Fisher Scientific (2 mentions) Aqua live dead dye, by Thermo Fisher Scientific (2 mentions) Cd27 apc, by Thermo Fisher Scientific (2 mentions) Cd19 fitc, by BD (2 mentions) Rnaseout, by Thermo Fisher Scientific (2 mentions) Cd38 pe, by BD (2 mentions)

2 protocols using cd3 cy7pe

1

Single-cell sorting of plasmablasts

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were freshly isolated from blood samples collected 7 days after PfSPZ immunization and stained for viability with Aqua Live/Dead dye(Invitrogen) followed by surface-staining for the following markers: CD20-Cy7APC (BD Bioscience), CD19-FITC (BD Bioscience), CD3-Cy7PE (BD Bioscience), CD38-PE (BD Bioscience), and CD27-APC (ThermoFisher). Plasmablasts were gated as live, CD3-CD20-CD19+CD27+CD38+ and were sorted as single cells into 96-well PCR plates containing 20 μl/well of RT reaction buffer that included 5 μl of 5× First strand cDNA buffer, 0.5 μl of RNAseOut (Invitrogen), 1.25 μl of DTT, 0.0625 μl of Igepal and 13.25 μl of dH2O (Invitrogen) as previously described46 (link).
Kisalu N.K., Idris A.H., Weidle C., Flores-Garcia Y., Flynn B.J., Sack B.K., Murphy S., Schön A., Freire E., Francica J.R., Miller A.B., Gregory J., March S., Liao H.X., Haynes B.F., Wiehe K., Trama A.M., Saunders K.O., Gladden M.A., Monroe A., Bonsignori M., Kanekiyo M., Wheatley A.K., McDermott A.B., Farney S.K., Chuang G.Y., Zhang B., Natasha K.C., Chakravarty S., Kwong P.D., Sinnis P., Bhatia S.N., Kappe S.H., Sim B.K., Hoffman S.L., Zavala F., Pancera M, & Seder R.A. (2018). A human monoclonal antibody prevents malaria infection and defines a new site of vulnerability on Plasmodium falciparum circumsporozoite protein. Nature medicine, 24(4), 408-416.
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2

Single-cell sorting of plasmablasts

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were freshly isolated from blood samples collected 7 days after PfSPZ immunization and stained for viability with Aqua Live/Dead dye(Invitrogen) followed by surface-staining for the following markers: CD20-Cy7APC (BD Bioscience), CD19-FITC (BD Bioscience), CD3-Cy7PE (BD Bioscience), CD38-PE (BD Bioscience), and CD27-APC (ThermoFisher). Plasmablasts were gated as live, CD3-CD20-CD19+CD27+CD38+ and were sorted as single cells into 96-well PCR plates containing 20 μl/well of RT reaction buffer that included 5 μl of 5× First strand cDNA buffer, 0.5 μl of RNAseOut (Invitrogen), 1.25 μl of DTT, 0.0625 μl of Igepal and 13.25 μl of dH2O (Invitrogen) as previously described46 (link).
Kisalu N.K., Idris A.H., Weidle C., Flores-Garcia Y., Flynn B.J., Sack B.K., Murphy S., Schön A., Freire E., Francica J.R., Miller A.B., Gregory J., March S., Liao H.X., Haynes B.F., Wiehe K., Trama A.M., Saunders K.O., Gladden M.A., Monroe A., Bonsignori M., Kanekiyo M., Wheatley A.K., McDermott A.B., Farney S.K., Chuang G.Y., Zhang B., Natasha K.C., Chakravarty S., Kwong P.D., Sinnis P., Bhatia S.N., Kappe S.H., Sim B.K., Hoffman S.L., Zavala F., Pancera M, & Seder R.A. (2018). A human monoclonal antibody prevents malaria infection and defines a new site of vulnerability on Plasmodium falciparum circumsporozoite protein. Nature medicine, 24(4), 408-416.
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