miR-15b mimics, miRNA mimic negative control (miRcon), miR-15b inhibitors (anti-miR-15b), and miRNA inhibitor negative control (anti-miR-con) were purchased from GenePharma (Shanghai, China). Synthetic miR-15b mimics, miR-con, miR-15b inhibitors, and anti-miR-con were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Small interfering RNA (siRNA) vectors targeting BACE1 were purchased from GenePharma (Shanghai, China). Transfection was conducted using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions.
Anti mir 15b
Manufactured by GenePharma
Sourced in China
Anti-miR-15b is a laboratory reagent designed to inhibit the function of microRNA-15b. It is used in research settings to study the role of microRNA-15b in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Anti mir con, by GenePharma (1 mentions)
Mir con, by Thermo Fisher Scientific (1 mentions)
Anti mir con, by Thermo Fisher Scientific (1 mentions)
Gsk 3β, by Santa Cruz Biotechnology (1 mentions)
Mir 15b mimic, by GenePharma (1 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions)
Mcl 1 sirna, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti mir 15b
1
Modulating miR-15b Expression Using Mimics and Inhibitors
2
Targeted Knockdown and Overexpression of GSK-3β and MCL-1 in NSCLC
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To knockdown the GSK-3β and MCL-1 directly, GSK-3β and MCL-1 siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). To overexpress the GSK-3β and MCL-1 directly, the open reading frame of GSK-3β and MCL-1 without the 3'-UTR was amplified and cloned into the pcDNA3.1 vector (Life Technologies), respectively. miR-15b mimics (5'-UAGCAGCACAU-CAUGGUUUACA-3'), anti-miR-15b (5'-UGUAAAC-CAUGAUGUGCUGCUA-3') and negative control oligonucleotide (NCO, 5'-UGCACAGUUUAACCAGGAUUCA-3') were purchased from Genepharma Company (Shanghai, China). For transfection, plasmid (2 μg/mL) and RNA oligonucleotides (50 pmol/mL) were transfected into the NSCLC cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer's instruction. Cells were collected and used for the following experiments 24 h after transfection.
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