ECFCs were transfected with siGENOME SMARTpool human HIF-1α, HIF-2α and MT1-MMP (Dharmacon, Lafayette, CO) following the manufacture’s protocol, as previously described.26 (link), 51 (link), 52 (link) Cells were seeded on a six-well plate and treated with 100 nM siRNA. We performed mRNA analysis after 24 hours and used confirmed transfected cells for experiments after 48 hours.
Sigenome smartpool human hif 1α
Manufactured by Horizon Discovery
The SiGENOME SMARTpool human HIF-1α is a collection of small interfering RNAs (siRNAs) designed to target the HIF-1α gene in human cells. The HIF-1α gene encodes the hypoxia-inducible factor 1-alpha subunit, a transcription factor that plays a key role in the cellular response to low oxygen conditions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sigenome smartpool human hif 1α
1
Silencing Transcription Factors in ECFCs
2
Silencing Transcription Factors in ECFCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ECFCs were transfected with siGENOME SMARTpool human HIF-1α, HIF-2α and MT1-MMP (Dharmacon, Lafayette, CO) following the manufacture’s protocol, as previously described.26 (link), 51 (link), 52 (link) Cells were seeded on a six-well plate and treated with 100 nM siRNA. We performed mRNA analysis after 24 hours and used confirmed transfected cells for experiments after 48 hours.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!