Uv 1601pc spectrometer

Optical rotations were measured on a Perkin-Elmer 343 polarimeter (Perkin Elmer, Waltham, MA, USA). UV spectra were recorded on a Shimadzu UV-1601PC spectrometer (Shimadzu Corporation, Kyoto, Japan) in methanol. CD spectra were measured with a Chirascan-Plus CD Spectrometer (Leatherhead, UK) in methanol. NMR spectra were recorded in CD₃OD and CDCl₃, on a Bruker DPX-500 (Bruker BioSpin GmbH, Rheinstetten, Germany) and a Bruker DRX-700 (Bruker BioSpin GmbH, Rheinstetten, Germany) spectrometer, using TMS as an internal standard. HRESIMS spectra were measured on a Maxis impact mass spectrometer (Bruker Daltonics GmbH, Rheinstetten, Germany).
Low-pressure liquid column chromatography was performed using Si gel L (50/100 μm, Imid, Russia) and Gel ODS-A (12 nm, S – 75 um, YMC Co, Ishikawa, Japan). Plates precoated with Si gel (5–17 μm, 4.5 × 6.0 cm, Imid) and Si gel60 RP-18 F₂₅₄S (20 × 20 cm, Merck KGaA, Germany) were used for thin-layer chromatography. Preparative HPLC was carried out on aAgilent 1100 chromatograph (Agilent Technologies, USA) using a YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 × 250 mm), YMC ODS-A (YMC Co., Ishikawa, Japan) (5 µm, 4.6 × 250 mm) and Supelco Discovery C-18 (5 μm, 250 × 4.6 mm) columns with a Agilent 1100 refractometer (Agilent Technologies, Santa Clara, CA, USA).

Optical rotations were measured on a Perkin-Elmer 343 polarimeter in MeOH. UV spectra were recorded on a Shimadzu UV-1601PC spectrometer in MeOH. ECD spectra were measured on a Chirascan-Plus CD Spectrometer in MeOH. ¹H and ¹³C NMR spectra were recorded in aceton-d₆ on a Bruker Avance-300, Avance-500 and Avance III-700 spectrometers (Bruker BioSpin GmbH) operating at 300 and 75, 500 and 125 MHz and 700 and 176 MHz, respectively. HRESIMS spectra were obtained on a Bruker maXis Impact II mass spectrometer (Bruker Daltonics GmbH). Low-pressure liquid column chromatography was performed using C₁₈-SiO₂ Gel ODS-A (12 nm, S—75 um, YMC Co., Ishikawa, Japan) and a Buchi B-688 Chromatography Pump on a Buchi glass column using Si gel KSK (50/100 μm, Imid Ltd., Russia). Plates precoated with Si gel (5–17 μm, 4.5 × 6.0 cm, Imid Ltd., Russia) and Si gel 60 RP-18 F254S (20 × 20 cm, Merck KGaA, Germany) were used for thin-layer chromatography. Preparative HPLC was carried out on a Shimadzu LC-20 (Shimadzu, Kyoto, Japan) and Agilent 1100 (Agilent Technologies, Santa Clara, CA, USA) chromatographs using a Shimadzu RID-20A and Agilent 1100 refractometers and YMC ODS-AM (YMC Co, 5 μm, 250 × 10 mm), Ultrasphere Si (5 μm, 250 × 4.6 mm), and Hydro-RP (Phenomenex, 4 μm, 250 × 10 mm) columns.