Ethyl acetate

Styrene, maleic anhydride (MA), p-divinylbenzene (DVB) and 2,2’-azo-bis(isobutyronitrile) (AIBN) were purchased from Sigma-Aldrich (Prague, Czech Republic). Styrene was distilled, MA was resublimed and AIBN was recrystallized from methanol prior to use. Ethyl acetate, ethanol and acetone of analytical grade were obtained from Lach-Ner (Neratovice, Czech Republic). Acetonitrile (ACN) and water of HPLC grade were purchased from VWR (Prague, Czech Republic). Phosphate buffered saline (PBS) solution (pH 7.4) was prepared from tablets supplied by Sigma-Aldrich (Prague, Czech Republic) according to the manufacturer’s instructions. Fetal bovine serum (FBS) was purchased from Sigma-Aldrich (Prague, Czech Republic). Cell culture medium (CellGRO) and AlamarBlue Assay were purchased from ThermoFisher Scientific (Prague, Czech Republic), 24-well plates were from TPP (Prague, Czech Republic).

FeCl₃·6H₂O, FeCl₂·4H₂O, trisodium citrate dihydrate, glycine methyl ester hydrochloride, triethylamine, methacryloyl chloride, 1-phenyl-3-pyrazolidinone, NH₂OH·HCl, 2,2′-azobis(2-methylpropionitrile) (AIBN), 4-Cyano-4-(phenylcarbonothioylthio)pentanoic acid (CTPA), phosphate buffered saline (PBS), 1,2-diaminoethane and silica gel (pore size 60 Å) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aqueous ammonia, hydrogen peroxide, HCl, 1,2-dichloroethane (DCE), NaCl, methanol, ethyl acetate, NaOH and diethyl ether were purchased from Lach-Ner, Neratovice, Czech Republic. Albumin was obtained from Serva (Heidelberg, Germany). Sephadex^® G-20 was purchased from GE Healthcare (Chicago, IL, USA). Aqueous solutions of FeCl₃·6H₂O and FeCl₂·4H₂O were purified by centrifugation for 10 min at 4400 rpm. 1,2-Dichlorethane, triethylamine, 2,2′-azobis(2-methylpropionitrile) (AIBN) and 4-Cyano-4-(phenylcarbonothioylthio)pentanoic acid (CTPA) were distilled before use. Ethanol was rectified. N-(2-hydroxypropyl)methacrylamide) (HPMA) was prepared according to a literature procedure [69 (link)]. All aqueous solutions in this study were prepared from ultrapure Q-water (18.2 MΩ) ultrafiltered on a Milli-Q Gradient A10 system (Milipore, Molsheim, France).

Butyl methacrylate (BMA), hydroquinone, itaconic acid (IA), methanol, methyl methacrylate (stab. with topanol; MMA), styrene (stabilized; STY), and toluene-4-sulfonic acid monohydrate (PTSA) were supplied from Thermo Fisher Scientific. Diethylene glycol (DEG), sulfuric acid (96%), sodium sulfate (anhydrous) and tetrahydrofuran (THF) were supplied from Penta. Ethyl acetate, butanol, and toluene were supplied from Lach-Ner. Dibutyltin oxide (DBTO) was obtained from PMC Group. Butanone peroxide (32% in a mixture of dimethyl phthalate and dicetone alcohol; MEKP) was obtained from Stachema. Oxidovanadium(iv) dibutylphosphate (10.6% V) was synthesized as described elsewhere.⁴⁵ Colorless PET bottles without cups and labels were washed with distilled water, cut to 2 × 2 cm² flakes, and dried in an oven at 50 °C for 1 h and characterized by measurements of intrinsic viscosity [η]. The value of 0.77 dL g⁻¹ was determined using an Ubbelohde viscometer in a 3/2 mixture (w/w) of phenol/1,1,2,2-tetrachloroethane at 30 °C.