Rt 6100 microplate reader

Plasma nitrite (NO₂-) and nitrate (NO₃) levels were measured by the Griess method, as previously described [5 (link)]. Briefly, 100 µL of each sample and each standard were pipetted into separate wells. Fifty microliters of 2% sulfanilamide (in 5% HCl) were added, followed by 50 µL of 0.1% N-(1 naphthyl) ethylenediamine dihydrochloride (NEDD) in deionized water. After incubation, the absorbance of the reaction mix was read at 540 nm by spectrophotometer (RT-6100 Microplate Reader, Rayto).

According to the provided instructions, the concentrations of cytokines (TNF-α, IL-1β), prostaglandin E2 (PGE-2), and L-Gln in the supernatant of cultured chondrocytes and rat serum were determined using ELISA kits (R&D Systems), while the nitric oxide (NO) concentration was detected with Griess reagent. For ELISA and the Griess reagent, the absorbances were measured at 450 and 540 nm, respectively, using a Rayto RT-6100 microplate reader.

The OGTT procedure was conducted following ADA recommendations (21 (link)). Blood glucose was measured in plasma using a blood glucose detector with Kyoto U-Test (Kyoto, Japan). Blood samples were collected from all subjects before and after Tai Chi intervention. Fasting for more than 8 h was required, and 5 ml of venous blood was taken on an empty stomach from 7:00 to 9:00 the next morning. Fasting insulin (FINs), total cholesterol (TC), triglyceride (TG), low-density cholesterol (LDL-C), high-density cholesterol (HDL-C), FBG, and other biochemical indexes were determined by Hitachi 7600 automatic biochemical analyzer. HbA_lC was determined by Hitachi 7170A automatic glycated hemoglobin analyzer. The homeostatic model assessment for insulin resistance was calculated to evaluate insulin resistance: homeostatic model assessment for insulin resistance (HOMA-IR) = (FBG×FINs)/22.5. The concentrations of NF-κB, ROS, NLRP3, ASC, Caspase-1, GSDMD, IL-1β, and IL-18 in serum of all subjects were detected by enzyme-linked immunosorbent assay (ELISA). The ELISA kits were purchased from Sin-Troch (China). The instrument was Rayto and RT-6100 microplate reader, and the operation was completed according to the kit instructions. The operation is completed according to the operating instructions.

Similarly, whole shoots from 6-week-old SD ecotype and XJ ecotype seedlings were collected, and the concentration of 1-aminocyclopropane-1-carboxylate oxidase (ACO) was determined by Enzyme-Linked Immunosorbent Assay (Shanghai Enzyme-Linked Biotechnology Co., Ltd., Shanghai, China) using the RT-6100 Microplate Reader (Rayto, Shenzhen, China).