Log In Sign up
The largest database of trusted experimental protocols

Infinium humanmethylation450 bead arrays

Manufactured by Illumina
Visit Supplier

The Infinium HumanMethylation450 bead arrays are a high-throughput DNA methylation profiling platform developed by Illumina. The arrays interrogate over 450,000 CpG sites across the human genome, providing a comprehensive analysis of DNA methylation patterns.

Automatically generated - may contain errors

Lab products found in correlation

Ez dna methylation kit, by Zymo Research (5 mentions)

Close spelling variants of this product

Infinium HumanMethylation450 array Infinium HumanMethylation450 HumanMethylation450 array Infinium arrays

5 protocols using infinium humanmethylation450 bead arrays

1

Profiling CHKA, ETNK1, and ETNK2 Methylation

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Analysis of methylation of CHKA, ETNK1, and ETNK2 genes was performed as previously described [6 (link)]. Briefly, genomic DNA was isolated; bisulfite was converted using the EZ DNA MethylationKit (Zymo Research) and processed on Infinium Human Methylation 450 bead arrays (Illumina). Probes that were significantly different between IDHwt and IDHmut cells were identified using the Limma (moderated t test) approach as described previously [23 (link), 24 (link)]. The ratio between methylated probe intensity and total probe intensity, which can be interpreted as the percentage of methylation, was designated as the beta (β) value and used to calculate the Δβ values (the difference between IDHmut and IDHwt cells) for each gene.
Viswanath P., Radoul M., Izquierdo-Garcia J.L., Luchman H.A., Gregory Cairncross J., Pieper R.O., Phillips J.J, & Ronen S.M. (2018). Mutant IDH1 gliomas downregulate phosphocholine and phosphoethanolamine synthesis in a 2-hydroxyglutarate-dependent manner. Cancer & Metabolism, 6, 3.
+ Open protocol
+ Expand
2

DNA Methylation Profiling on Infinium Arrays

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA from 70 samples was bisulfite converted using the EZ DNA Methylation Kit (Zymo Research) and processed on Infinium HumanMethylation450 bead arrays (Illumina Inc.) according to the manufacturer's protocol. Probe-level signals for individual CpG sites were subject to both background and global dye-bias correction (Triche et al., 2013 (link)). Probes that map to regions with known germline polymorphisms, to multiple genomic loci (Price et al., 2013 (link)), or to either sex chromosome were filtered out. Descriptions of further analyses are provided in Supplemental Experimental Procedures.
Mazor T., Pankov A., Johnson B.E., Hong C., Hamilton E.G., Bell R.J., Smirnov I.V., Reis G.F., Phillips J.J., Barnes M.J., Idbaih A., Alentorn A., Kloezeman J.J., Lamfers M.L., Bollen A.W., Taylor B.S., Molinaro A.M., Olshen A.B., Chang S.M., Song J.S, & Costello J.F. (2015). DNA methylation and somatic mutations converge on cell cycle and define similar evolutionary histories in brain tumors. Cancer cell, 28(3), 307-317.
+ Open protocol
+ Expand
3

Genome-wide DNA Methylation Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA from JMML patients was extracted using standard methods from bone marrow, splenic tissue or peripheral blood mononuclear cells or granulocytes obtained at diagnosis. Genomic DNA was bisulfite converted using the EZ DNA Methylation Kit (Zymo Research) and processed on Infinium HumanMethylation450 bead arrays (Illumina Inc.) according to the manufacturer’s protocol.
Stieglitz E., Mazor T., Olshen A.B., Geng H., Gelston L.C., Akutagawa J., Lipka D.B., Plass C., Flotho C., Chehab F.F., Braun B.S., Costello J.F, & Loh M.L. (2017). Genome-wide DNA methylation is predictive of outcome in juvenile myelomonocytic leukemia. Nature Communications, 8, 2127.
+ Open protocol
+ Expand
4

Genome-wide DNA Methylation Profiling of ASXL1 Mutations

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was bisulfite converted using the EZ DNA Methylation Kit (Zymo Research) and processed on Infinium HumanMethylation450 bead arrays (Illumina Inc.) according to the manufacturer's protocol. Probe-level signals for individual CpG sites were subject to both background and global dye-bias correction74 (link). Probes that map to regions with known germline polymorphisms, to multiple genomic loci, or to either sex chromosome were filtered out. Two-way unsupervised hierarchical clustering was performed using Euclidean distance and Ward linkage on the 1,486 most variable CpG sites across the cohort, with variability ranked by standard deviation. Four subjects with clonal ASXL1 mutations at diagnosis were included for analysis. An additional seven patients were chosen as controls as they had similar mutational profiles compared to the subjects except they did not possess any known genetic mutations in epigenetic regulating genes (Supplementary Figure 6).
Stieglitz E., Taylor-Weiner A.N., Chang T.Y., Gelston L.C., Wang Y.D., Mazor T., Esquivel E., Yu A., Seepo S., Olsen S., Rosenberg M., Archambeault S.L., Abusin G., Beckman K., Brown P.A., Briones M., Carcamo B., Cooper T., Dahl G.V., Emanuel P.D., Fluchel M.N., Goyal R.K., Hayashi R.J., Hitzler J., Hugge C., Liu Y.L., Messinger Y.H., Mahoney DH J.r., Monteleone P., Nemecek E.R., Roehrs P.A., Schore R.J., Stine K.C., Takemoto C.M., Toretsky J.A., Costello J.F., Olshen A.B., Stewart C., Li Y., Ma J., Gerbing R.B., Alonzo T.A., Getz G., Gruber T., Golub T., Stegmaier K, & Loh M.L. (2015). The Genomic Landscape of Juvenile Myelomonocytic Leukemia. Nature genetics, 47(11), 1326-1333.
+ Open protocol
+ Expand
5

Genome-wide DNA Methylation Profiling of ASXL1 Mutations

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was bisulfite converted using the EZ DNA Methylation Kit (Zymo Research) and processed on Infinium HumanMethylation450 bead arrays (Illumina Inc.) according to the manufacturer's protocol. Probe-level signals for individual CpG sites were subject to both background and global dye-bias correction74 (link). Probes that map to regions with known germline polymorphisms, to multiple genomic loci, or to either sex chromosome were filtered out. Two-way unsupervised hierarchical clustering was performed using Euclidean distance and Ward linkage on the 1,486 most variable CpG sites across the cohort, with variability ranked by standard deviation. Four subjects with clonal ASXL1 mutations at diagnosis were included for analysis. An additional seven patients were chosen as controls as they had similar mutational profiles compared to the subjects except they did not possess any known genetic mutations in epigenetic regulating genes (Supplementary Figure 6).
Stieglitz E., Taylor-Weiner A.N., Chang T.Y., Gelston L.C., Wang Y.D., Mazor T., Esquivel E., Yu A., Seepo S., Olsen S., Rosenberg M., Archambeault S.L., Abusin G., Beckman K., Brown P.A., Briones M., Carcamo B., Cooper T., Dahl G.V., Emanuel P.D., Fluchel M.N., Goyal R.K., Hayashi R.J., Hitzler J., Hugge C., Liu Y.L., Messinger Y.H., Mahoney DH J.r., Monteleone P., Nemecek E.R., Roehrs P.A., Schore R.J., Stine K.C., Takemoto C.M., Toretsky J.A., Costello J.F., Olshen A.B., Stewart C., Li Y., Ma J., Gerbing R.B., Alonzo T.A., Getz G., Gruber T., Golub T., Stegmaier K, & Loh M.L. (2015). The Genomic Landscape of Juvenile Myelomonocytic Leukemia. Nature genetics, 47(11), 1326-1333.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!