Multi function microplate reader

Manufactured by PerkinElmer

Sourced in United States

The Multi-function microplate reader is a versatile instrument designed for rapid and accurate analysis of samples in microplate format. It can perform a variety of detection methods, including absorbance, fluorescence, and luminescence measurements, to support a wide range of applications in life science research and clinical diagnostics.

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Lab products found in correlation

Luciferase based enhanced atp assay kit, by Beyotime (2 mentions) Cell counting kit 8, by Dojindo Laboratories (1 mentions) Flow cytometry, by Beyotime (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Dcfh da, by Nanjing Jiancheng (1 mentions) Cck 8, by Merck Group (1 mentions) Il 1β, by PerkinElmer (1 mentions) Il 18, by R&D Systems (1 mentions) Il 1β, by RayBiotech (1 mentions)

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Microplate reader

7 protocols using multi function microplate reader

Cell Viability Assay with CCK-8

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The viability of cells was determined by Cell Counting Kit, CCK-8 (Dojindo, Japan). Briefly, cells in the exponential growth phase were harvested; suspension of 5 × 10³ single cells were seeded in each well of 96-well plates and incubated at 37°C overnight. The cultures were added respectively with DMSO as vehicle control and the compound N69B of different concentrations, and grown for 24, 48 or 72 h. The cells were added with CCK-8 and incubated with for 1–2 h.
The relative number of viable cells was determined by measuring the absorbance with Multi-function microplate reader (PerkinElmer, EnSpire, America) at a wavelength of 450 nm. Triplicates were run in three independent tests.

Wu J., Huang Y., Xie Q., Zhang J, & Zhan Z. (2020). A novel bis-aryl urea compound inhibits tumor proliferation via cathepsin D-associated apoptosis. Anti-Cancer Drugs, 31(5), 500-506.

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Mitochondrial Membrane Potential and ATP Measurement

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JC-1 mitochondrial membrane potential measurement kits (Beyotime, China) were used to measure mitochondrial membrane potential (ΔΨm). Briefly, TM3 cells (2 × 10⁵) were resuspended in 0.5 mL JC-1 working solution and incubated at 37 °C for 20 min. The cells were washed with buffer twice and then analyzed by flow cytometry (BD Biosciences, USA). A luciferase-based enhanced ATP assay kit (Beyotime, China) was used to determine ATP levels. Briefly, the cells were washed with cold PBS in 6-well plates and lysed immediately in 200 μL lysis buffer on ice. The lysate was centrifuged at 12,000 g for 5 min. 20 μL of the supernatant was added into a 96-well plate containing 100 μL ATP detection working solution. The luminescence was detected by a multi-function microplate reader (Perkinelmer, USA). The protein concentration of each group and an ATP standard curve were used to calibrate the ATP levels in the cells. The cellular ATP levels were presented as the percentage of the level observed in the control group.

Yi L., Shang X.J., Lv L., Wang Y., Zhang J., Quan C., Shi Y., Liu Y, & Zhang L. (2022). Cadmium-induced apoptosis of Leydig cells is mediated by excessive mitochondrial fission and inhibition of mitophagy. Cell Death & Disease, 13(11), 928.

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Mitochondrial Membrane Potential and ATP Assay

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A JC-1 MMP measurement kit (Beyotime, Shanghai, China) was used to measure MMP (ΔΨm). Briefly, the indicated cells (2×10⁵) were resuspended in 0.5 mL of JC-1 working solution and incubated for 20 min. The cells were washed and analyzed by flow cytometry (Biosciences, Franklin Lakes, NJ).
A luciferase-based enhanced ATP assay kit (Beyotime, Shanghai, China) was used to determine ATP levels. Briefly, the indicated cells were lysed and centrifuged at 12,000× g for 5 min. The supernatant was added to a 96-well plate containing ATP detection working solution. Luminescence was detected by a multifunction microplate reader (Perkin Elmer, USA). The protein concentration of each group and an ATP standard curve were used to calibrate the ATP levels in the cells.

Tang D., Zhao L., Huang S., Li W., He Q, & Wang A. (2024). Mitochondrial outer membrane protein MTUS1/ATIP1 exerts antitumor effects through ROS-induced mitochondrial pyroptosis in head and neck squamous cell carcinoma. International Journal of Biological Sciences, 20(7), 2576-2591.

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Measuring Intracellular ROS and Mitochondrial Superoxide

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2,7-Dichlorofuorescin Diacetate (DCFH-DA) (Nanjing Jiancheng Bioengineering Institute, China) and MitoSOX Red (Thermo Fisher Scientific, USA) were used to measure intracellular ROS and mitochondrial superoxide production, respectively. TM3 cells exposed to cadmium for 24 h were counted and incubated in 10 μM DCFH-DA and 2.5 μM MitoSOX at 37 °C for 30 min in the dark. The cells were analyzed by a multi-function microplate reader (Perkinelmer, USA).

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Evaluating H2O2 Impact on RPE Cells

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The effects of H₂O₂ on the viability of hRPE and ARPE-19 cells were explored by cell counting kit-8 (CCK-8, Catalog No. 96992, Sigma-Aldrich) according to the manufacturer’s instructions. Briefly, approximately 4000 hRPE or ARPE-19 cells were seeded in 96-well plates and allowed to adhere for 12 h. Next, gradient concentrations of H₂O₂ (or supplement with 0, 5, or 10 mM lutein) were added and incubated with the cells for another 48 h before WST-8 addition to each well. After incubation for another 1 h, absorbance was measured using a multifunction microplate reader (PerkinElmer, Waltham, MA). hRPE or ARPE-19 cells cultured without any treatment were used as control.

Jin X., Liu J., Wang W., Li J., Liu G., Qiu R., Yang M., Liu M., Yang L., Du X, & Lei B. (2022). Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium. Genomics, Proteomics & Bioinformatics, 20(4), 633-647.

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Quantifying Cytokine Levels in Rat Serum

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ELISA kits (Boster Biological Technology, Wuhan, China) were used to detect the levels of IL-1β, TNF-α, IL-10, and IL-18 in rat serum. The absorbance (OD) was measured using a multi-function microplate reader (PerkinElmer, Waltham, MA, USA) at 450 nm wavelength, and the levels of targeted molecules in the serum were calculated using a respective standard curve.

Zhang M., Wen Y., Liang P., Yang C., Tu H., Wei J., Du J., Zhan T., Liang S., Li G, & Gao Y. (2023). Imperatorin Improves Obesity-Induced Cardiac Sympathetic Nerve Injury Mediated by P2X4 Receptor in Stellate Sympathetic Ganglion. International Journal of Molecular Sciences, 24(1), 783.

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IL-1β and IL-18 quantification in ox-LDL-induced ARPE-19 cells

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After pretreatment with 1 μM A740003 for 2 hours, ARPE-19 cells were induced with ox-LDL for 24 hours. Then, supernatants of all groups were collected and centrifuged to detect the concentrations of IL-1β (RayBiotech, Norcross, GA, USA) and IL-18 (R&D Systems, Minneapolis, CA, USA) by using human ELISA kits according to the manufacturers' protocols. The concentrations of IL-1β and IL-18 were calculated according to optical density measured at 450 nm by subtracting the optical density measured at 540 or 570 nm using a multifunction microplate reader (PerkinElmer, Waltham, MA, USA).

Yang M., Qiu R., Wang W., Liu J., Jin X., Li Y., Li L, & Lei B. (2021). P2X7 Receptor Antagonist Attenuates Retinal Inflammation and Neovascularization Induced by Oxidized Low-Density Lipoprotein. Oxidative Medicine and Cellular Longevity, 2021, 5520644.

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