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Mouse anti p62 sqstm1 d 3

Manufactured by Cell Signaling Technology
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Mouse anti-p62/SQSTM1 (D-3) is a primary antibody that recognizes the p62/SQSTM1 protein. p62/SQSTM1 is a multifunctional protein involved in various cellular processes.

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Lab products found in correlation

Mouse anti β actin ac 74, by Merck Group (2 mentions) Rabbit anti beclin1 antibody, by Cell Signaling Technology (2 mentions) A5316, by Merck Group (2 mentions) Mouse anti n cadherin, by BD (2 mentions) Rabbit anti lc3b, by Cell Signaling Technology (2 mentions) Mouse anti cd44 clone 156 c11, by Cell Signaling Technology (2 mentions) Mouse anti e cadherin, by BD (2 mentions)

2 protocols using mouse anti p62 sqstm1 d 3

1

Comprehensive Western Blotting Approach

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed as described previously18 (link), 22 (link) using the following primary antibodies: rabbit anti-LC3B (1:1000; 2775; Cell Signaling Technology), mouse anti-p62/SQSTM1 (D-3) (1:1000; sc-28359; Cell Signaling Technology), rabbit anti-Beclin1 antibody (1:1000; 3738; Cell Signaling Technology), mouse anti-E-cadherin (1:10000; 610182; BD Biosciences), mouse anti-N-cadherin (1:1000; 610921; BD Biosciences), and mouse anti-β-Actin (AC-74) (1:10000; A5316; Sigma-Aldrich). To detect CD44, we utilized mouse anti-CD44 clone 156-C11 (1:1000; 3570; Cell Signaling Technology) which recognizes an epitope in the amino-terminal extracellular region of CD4448 , allowing for detection of the standard isoform of CD44 as well as variant CD44 isoforms. CD44v6 expression was evaluated via mouse anti-CD44v6 clone 2F10 (1:500; BBA13 R&D Systems). Densitometry was performed with Image J (National Institutes of Health).
Whelan K.A., Chandramouleeswaran P.M., Tanaka K., Natsuizaka M., Guha M., Srinivasan S., Darling D.S., Kita Y., Natsugoe S., Winkler J.D., Klein-Szanto A.J., Amaravadi R.K., Avadhani N.G., Rustgi A.K, & Nakagawa H. (2017). Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance. Oncogene, 36(34), 4843-4858.
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2

Comprehensive Western Blotting Approach

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed as described previously18 (link), 22 (link) using the following primary antibodies: rabbit anti-LC3B (1:1000; 2775; Cell Signaling Technology), mouse anti-p62/SQSTM1 (D-3) (1:1000; sc-28359; Cell Signaling Technology), rabbit anti-Beclin1 antibody (1:1000; 3738; Cell Signaling Technology), mouse anti-E-cadherin (1:10000; 610182; BD Biosciences), mouse anti-N-cadherin (1:1000; 610921; BD Biosciences), and mouse anti-β-Actin (AC-74) (1:10000; A5316; Sigma-Aldrich). To detect CD44, we utilized mouse anti-CD44 clone 156-C11 (1:1000; 3570; Cell Signaling Technology) which recognizes an epitope in the amino-terminal extracellular region of CD4448 , allowing for detection of the standard isoform of CD44 as well as variant CD44 isoforms. CD44v6 expression was evaluated via mouse anti-CD44v6 clone 2F10 (1:500; BBA13 R&D Systems). Densitometry was performed with Image J (National Institutes of Health).
Whelan K.A., Chandramouleeswaran P.M., Tanaka K., Natsuizaka M., Guha M., Srinivasan S., Darling D.S., Kita Y., Natsugoe S., Winkler J.D., Klein-Szanto A.J., Amaravadi R.K., Avadhani N.G., Rustgi A.K, & Nakagawa H. (2017). Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance. Oncogene, 36(34), 4843-4858.
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