Anhydrous methanol

Sophocarpine (purity ≧ 98%) and matrine (purity ≧ 98%) were purchased from Baoji Fangsheng Biological Development Co., Ltd. Pyrrolidine was purchased from Shanghai Macklin Biochemical Co., Ltd. Carbon disulfide, petroleum ether, and anhydrous methanol were purchased from Tianjin Damao Chemical Reagent Factory. Diethylamine was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Absolute ethanol, ethyl acetate and dichloromethane were purchased from Tianjin Kaitong Chemical Reagent Co., Ltd., Tianjin Beilian Fine Chemical Development Co., Ltd., and Tianjin Best Chemical Co., Ltd., respectively. Fetal bovine serum (FBS), antibiotics and phosphate buffer solution (PBS) were purchased from Gibco. Dimethyl sulfoxide (DMSO) and Thiazole blue (MTT) were purchased from SIGMA and Guangzhou Qiyun Biotechnology Co., Ltd., respectively.

Succinic acid (SA, 99.5%) and titanium isopropoxide (TTIP, 95%) were obtained from Shanghai Aladdin Biochemical Company (Shanghai, China). Ethylene glycol (EG, 98%), 1,6-hexanediol (HD, 98%), and decahydronaphthalene were purchased from Chengdu Aikeda Chemical Reagent Co., Ltd. (Chengdu, China). Chloroform was obtained from Shenyang Xinxing Reagent Factory (Shenyang, China). Anhydrous methanol was obtained from Tianjin Damao Chemical Reagent Factory (Tianjin, China). Cutinase was prepared following the procedure reported in our previous work (Hu et al., 2016 (link)). All chemicals and reagents were of analytical grade.