Ni 2 nitrilotriacetic acid agarose ni nta resin

Isopropyl-β-D-thiogalactopyranoside (IPTG), anhydrous sodium phosphate, and dibasic potassium phosphate were purchased from DOT Scientific. The buffer 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), glycerol, and imidazole were purchased from Fisher Scientific. Kanamycin was purchased from Teknova. Magnesium sulfate heptahydrate, L-β-homolysine and D-Lysine were purchased from Chem-Impex International. Ni(II)-nitrilotriacetic acid agarose (Ni-NTA) resin was purchased from Qiagen. L-Allylglycine was purchased from Cayman Chemical Company. 4,4,5,5-[²H₂]-L-Lysine (d₄-L-Lys) was purchased from Cambridge Isotope Laboratories. L-norleucine was purchased from Alfa Aesar. Trans-4,5-dehydro-DL-Lysine was purchased from Bachem. 6-Hydroxy-L-norleucine was purchased from Acrotein Chembio Inc. 4RS-Cl-DL-Lysine was purchased from Akos GmbH (Steinen, Germany). EZNA Plasmid DNA Mini Kit was purchased from Omega Bio-Tek. Oligonucleotide primers were purchased from Integrated DNA Technologies. Polymerase chain reaction (PCR) reagents, Q5 DNA Polymerase Master Mix, restriction enzymes, restriction enzyme buffers, and T4 DNA ligase were purchased from New England Biolabs. Escherichia coli (Ec) DH5α and Rosetta(DE3) competent cells were purchased from Novagen. ⁵⁷Fe⁰ was purchased from ISOFLEX, USA. All other chemicals were purchased from Millipore Sigma.

Isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from DOT Scientific. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), glycerol, and imidazole were purchased from Fisher Scientific. Kanamycin was purchased from Teknova. Sodium chloride (NaCl), sodium hydroxide (NaOH), ammonium chloride (NH₄Cl) and hydrochloric acid (HCl) were purchased from EMD Millipore. Ni(II)-nitrilotriacetic acid agarose (Ni-NTA) resin was purchased from Qiagen. 1H-pyrazole-1-(N-methylcarboxamidine)hydrochloride was purchased from Alfa Aesar. All other chemicals used were purchased from Sigma Aldrich. Escherichia coli (Ec) DH5α and BL21(DE3) competent cells were purchased from Novagen. ⁵⁷Fe⁰ was purchased from ISOFLEX, USA.