Phosphate-buffered saline (PBS) and VCM were purchased from Sigma-Aldrich (St. Louis, MO, USA) and RFB from Pharmacy Biotech AB (Uppsala, Sweden). The pure phospholipids, dimyristoyl phosphatidyl choline (DMPC), dimyristoyl phosphatidyl glycerol (DMPG), dipalmitoyl phosphatidyl choline (DPPC), dipalmitoyl phosphatidyl glycerol (DPPG), and distearoyl phosphatidyl ethanolamine covalently linked to poly(ethylene glycol)2000 (DSPE-PEG) were purchased from Lipoid (Ludwigshafen, Germany). Rhodamine covalently linked to phosphatidylethanolamine (Rho-PE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Thiazolyl blue tetrazolium bromide (MTT), crystal violet (CV), and glycerol were obtained from Panreac AppliChem, ITW Reagents (Darmstadt, Germany). Culture media Mueller–Hinton Agar (MHA) and Mueller–Hinton Broth (MHB) were obtained from Oxoid, Ltd. (Basingstoke, UK) and Tryptic Soy Broth (TSB) from Biokar (Pantin, France). The fluorescent stain SYTO 9 was obtained from Molecular Probes (Eugene, OR, USA). D(+)-glucose monohydrate was acquired from Merck KGaA (Darmstadt, Germany). Dimethyl sulfoxide (DMSO) and ethanol absolute anhydrous were obtained from Carlo Erba Reagents S.A.S. (Val de Reuil, France). All other reagents were of analytical grade.
Dipalmitoylphosphatidylcholine
Manufactured by Lipoid
Sourced in Germany
Dipalmitoylphosphatidylcholine (DPPC) is a synthetic phospholipid widely used in laboratory research. It is the primary component of natural lung surfactant and plays a crucial role in maintaining the stability of alveoli in the lungs. DPPC is a valuable tool for researchers studying membrane structure, liposome formation, and other applications in the field of biology and biophysics.
Automatically generated - may contain errors
Lab products found in correlation
Chloroform, by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
Dimyristoyl phosphatidyl choline, by Lipoid (2 mentions)
Dimyristoyl phosphatidyl glycerol, by Lipoid (2 mentions)
Dipalmitoyl phosphatidyl glycerol, by Lipoid (2 mentions)
Mueller hinton broth (mhb), by Thermo Fisher Scientific (2 mentions)
Mueller hinton agar (mha), by Thermo Fisher Scientific (2 mentions)
Tryptic soy broth tsb, by Solabia (2 mentions)
Syto9, by Thermo Fisher Scientific (2 mentions)
Hydrogenated soy phosphatidylcholine, by Lipoid (2 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 ammonium salt dspe peg2000, by Lipoid (2 mentions)
Cholesterol, by Merck Group (2 mentions)
Distearoyl phosphatidyl ethanolamine, by Lipoid (1 mentions)
Dspe peg, by Lipoid (1 mentions)
D glucose monohydrate, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Carlo Erba (1 mentions)
Ethanol absolute anhydrous, by Carlo Erba (1 mentions)
4 2 hydroxyethyl piperazine 1 ethanesulfonic acid, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
10 protocols using dipalmitoylphosphatidylcholine
1
Characterization of Liposomal Formulations
2
Liposomal Doxorubicin Formulation
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Dipalmitoylphosphatidylcholine (DPPC); hydrogenated soy phosphatidylcholine (HSPC); 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000) were kind gifts from Lipoid GmbH (Germany). 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine (MSPC) were purchased from Avanti Polar lipid (Alabama, USA). Cholesterol, isopropanol, chloroform, methanol, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), and doxorubicin hydrochloride (Dox) were purchased from Sigma (UK). [14C] doxorubicin hydrochloride (14C-Dox) were purchased from PerkinElmer (UK). All chemical substances and solvents were used without further purification.
3
Isolation and Structural Elucidation of Guttiferone E
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Guttiferone E was isolated from the stem bark of Garcinia punctata; the isolation procedure and the structure elucidation including necessary analytical techniques for compound purity were previously described [10 (link)]. Dipalmitoylphosphatidylcholine (DPPC) and cholesterol (CHOL) were purchased from LIPOID, Germany. Penicillin/streptomycin/fungizone (PSF), fetal bovine serum (FBS), culture media, and [3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide] (MTT) were supplied by Capricorn Scientific, Germany. Lipopolysaccharide (LPS) (Escherichia coli 0111:B4), sodium nitrite, the Griess reagent, and curcumin (from Curcuma longa) were provided by Sigma, Germany. Lysis buffer was obtained from Promega, Germany.
4
Preparation and Characterization of DPPC-based Liposomes
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Dipalmitoyl phosphatidylcholine (DPPC) and methoxy-polyethylene glycol-derivatized di- stearoyl phosphatidylethanolamine (DSPE-mPEG2000) were obtained from Lipoid GmbH (Ludwigshafen, Germany). Fluorescein isothiocyanate (FITC), HU and cholesterol (Chol) were provided from Sigma-Aldrich (St. Louis, MO, USA), Sucrose, Chloroform, and methanol were prepared from Merck (Darmstadt, Germany). Polycarbonate screens were supplied from Northern Lipids (Vancouver, Canada). Streptomycin-penicillin-glutamine (S/P/G), SephadexG-50 were taken from Invitrogen (Carlsbad, CA, USA). All other chemicals were of analytical grade. As well, the BT-474 cell line was bought from the American Type Culture Collection.
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Dipalmitoyl phosphatidylcholine (DPPC) and methoxy-polyethylene glycol-derivatized di- stearoyl phosphatidylethanolamine (DSPE-mPEG2000) were obtained from Lipoid GmbH (Ludwigshafen, Germany). Fluorescein isothiocyanate (FITC), HU and cholesterol (Chol) were provided from Sigma-Aldrich (St. Louis, MO, USA), Sucrose, Chloroform, and methanol were prepared from Merck (Darmstadt, Germany). Polycarbonate screens were supplied from Northern Lipids (Vancouver, Canada). Streptomycin-penicillin-glutamine (S/P/G), SephadexG-50 were taken from Invitrogen (Carlsbad, CA, USA). All other chemicals were of analytical grade. As well, the BT-474 cell line was bought from the American Type Culture Collection.
5
Nano-formulations for Antimicrobial Delivery
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Levofloxacin (LEV) and vancomycin (VCM) were obtained from Sigma–Aldrich (St. Louis, MO, USA), and Rifabutin (RFB) from Pharmacy Biotech AB (Uppsala, Sweden). The pure phospholipids, dimyristoyl phosphatidyl choline (DMPC), dimyristoyl phosphatidyl glycerol (DMPG), dipalmitoyl phosphatidyl choline (DPPC), dipalmitoyl phosphatidyl glycerol (DPPG) and dioleoyl phosphatidyl ethanolamine (DOPE) were purchased from Lipoid (Ludwigshafen, Germany). Stearylamine (SA) was obtained from Sigma–Aldrich (St. Louis, MO, USA). Cholesteryl hemisuccinate (CHEMS) and rhodamine covalently linked to phosphatidylethanolamine (Rho-PE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Nuclepore Track-Etch Membranes were purchased from Whatman Ltd. (Maidstone, UK). Thiazolyl Blue Tetrazolium Bromide (MTT) and Crystal Violet (CV) were obtained from Panreac Applichem, ITW Reagents (Darmstadt, Germany). Culture media, Mueller-Hinton Agar (MHA) and Mueller-Hinton Broth (MHB) were obtained from Oxoid, Ltd. (Basingstoke, UK), Tryptic Soy Broth (TSB) from Biokar (Pantin, France) and Columbia Agar + 5% sheep blood (CA) from bioMérieux (Marcy l’Étoile, France). The SYTO 9 was obtained from Molecular Probes (Eugene, OR, USA). All the remaining chemicals used were of analytical grade.
6
Formulation and Characterization of Lidocaine Formulations
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Lidocaine was obtained as a kind gift from Shouguang Fukang Pharmacy Factory (Shandong, China). Pluronic® F-127 (poloxmer 407) was purchased from BASF (Hanover, Germany). Soybean phosphatidylcholine and dipalmitoyl phosphatidylcholine were kindly gifted by Lipoid GmbH (Ludwigshafen, Germany). Disodium hydrogen phosphate, potassium dihydrogen phosphate, sucrose, and chloroform were purchased from Shanghai Chemical Co. (Shanghai, China). Other chemicals were of reagents or analytical grade.
7
Synthesis and Characterization of Doxorubicin-Loaded Nanoparticles
8
Lipid-based Nanoparticle Formulation Protocol
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Soybean oil and Myrj™ s40 (PEGylated surfactant) were purchased from Croda, Chocques, France. Lipoid S75 and dipalmitoylphosphatidylcholine were from Lipoid GmbH, Ludwigshafen, Germany. Suppocire NB was from Gatefosse, Saint-Priest, France. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), cholesterol, 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG-2000) and 1,2-distearoyl-phosphatidylethanolamine-methyl-polyethyleneglycol conjugate-550 (DSPE-PEG550) were purchased from Avanti Polar Lipids, Inc. (AL, USA).
9
Preparation and Evaluation of Lipid Nanocarriers
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Soya phosphatidylcholine (SPC; Lipoid S-100), hydrogenated phosphatidylcholine (HSPC; Phospholipon 90H) and dipalmitoyl phosphatidylcholine (DPPC) were obtained from Lipoid, Switzerland. EMEM (Eagle's minimum essential medium), non-essential amino acid solution and L-glutamine (cell culture tested, 99.0 -101.0 %) were purchased from Lonza, Switzerland. Trypsin-EDTA solution, ethanol (absolute and 70%), 96-well plates (sterile with lids), 50 ml centrifuge tubes (sterile), tissue culture flask 75 cm 2 (sterile) and serological pipettes (sterile) were obtained from Fisher Scientific, UK. Cholesterol (Chol; ≥ 99%), glass vials (15 ml), poly-L-lysine (PLL) hydrobromide (molecular weight 30,000-70,000), dextran (molecular weight 5,000 approx.), Dimethyl sulfoxide (DMSO), thiazolyl blue tetrazolium bromide, Fetal bovine serum (FBS), Phosphate buffered saline (PBS) tablets, Trypan blue solution (0.4% liquid, sterile filtered), Syringe filters (0.2 and 0.45 µm), syringe needles and sterile pipette tip boxes were all purchased from Sigma Aldrich, UK. Human glioblastome cell line (U87-MG) and Human glial cell line (SVG-P12) were obtained from European collection of cell cultures, UK. Paclitaxel (PX) was purchased from ChemieTek, USA.
10
Liposomal Doxorubicin Formulation Development
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Materials Dipalmitoylphosphatidylcholine (DPPC); monostearoyl phosphatidylcholine (MSPC); hydrogenated soy phosphatidylcholine (HSPC); 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000) were purchased from Lipoid GmbH (Germany). Cholesterol, chloroform, methanol, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), and doxorubicin hydrochloride (Dox) were purchased from Sigma (UK). All chemical substances and solvents were used without further purification.
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