Log In Sign up
The largest database of trusted experimental protocols

Bradykinin elisa kit

Manufactured by Enzo Life Sciences
Visit Supplier
Sourced in United States

The Bradykinin ELISA kit is a laboratory tool designed to measure the concentration of bradykinin, a signaling peptide, in biological samples. This kit uses the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.

Automatically generated - may contain errors

Lab products found in correlation

Agilent 1200 lc system, by Agilent Technologies (1 mentions) Api 4000 mass spectrometer, by AB Sciex (1 mentions) Innotest elisa kit, by Fujirebio (1 mentions) Porcine brain l α phosphatidylserine, by Avanti Polar Lipids (1 mentions) Egg l α phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Angiotensin 2 elisa kit, by Enzo Life Sciences (1 mentions) Human il 8 platinum elisa, by Thermo Fisher Scientific (1 mentions) Human il 6 platinum elisa, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Bradykinin (3 citations)

5 protocols using bradykinin elisa kit

1

Bradykinin ELISA Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whey BK levels were measured using a commercially available ELISA kit (Bradykinin ELISA
kit, Inter-assay 6.8%CV, assay sensitivity 24.8 pg/mL; Enzo Life Sciences, Inc.,
Farmingdale, NY, USA).
HISAEDA K., NOHARA M., OHTA N., GOTO A., INOUE Y., HATA A, & SHINOZUKA Y. (2024). Effect of 2% meloxicam injection in Holstein dairy cows on acute clinical mastitis without systemic symptoms. The Journal of Veterinary Medical Science, 86(4), 374-380.
+ Open protocol
+ Expand
2

Quantifying Kinin Peptides in Viral Inactivated BAL Fluid

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lys-bradykinin and bradykinin were measured in viral inactivated BAL fluid samples using an enzyme-linked immunosorbent assay (ELISA) according to manufacturer instructions (Bradykinin ELISA kit; Enzo Life Sciences, cat. ADI-900-206). In addition, we measured kinins (Lys-bradykinin, bradykinin) and their metabolites (Lys-bradykinin-(1-8), bradykinin-(1-8), bradykinin-(1-7), and bradykinin-(1-5)) using a previously published and more sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) assay in collaboration with the University of Düsseldorf, Germany.18 ,19 (link) For this analysis, 100 µL of BAL fluid was purified by a customized weak cation exchange solid-phase extraction. The resulting eluate was evaporated under a gentle stream of nitrogen at 60°C on a shaker at 350 rpm. The dissolved residue was then analysed using an Agilent 1200 LC-system (Agilent Technologies, Ratingen, Germany) coupled to an API 4000 mass spectrometer (AB Sciex, Darmstadt, Germany). The respective quantification limits for the assessed kinin peptides were: 4·4 pg/mL for Lys-bradykinin, 6·7 pg/mL for bradykinin, 10·6 pg/mL for Lys-bradykinin-(1-8), 7·3 pg/mL for bradykinin-(1-8), 6·5 pg/mL for bradykinin-(1-7), and 22·8 pg/mL for bradykinin-(1-5).
Martens C.P., Van Mol P., Wauters J., Wauters E., Gangnus T., Noppen B., Callewaert H., Feyen J.H., Liesenborghs L., Heylen E., Jansen S., Pereira L.C., Kraisin S., Guler I., Engelen M.M., Ockerman A., Van Herck A., Vos R., Vandenbriele C., Meersseman P., Hermans G., Wilmer A., Martinod K., Burckhardt B.B., Vanhove M., Jacquemin M., Verhamme P., Neyts J, & Vanassche T. (2022). Dysregulation of the kallikrein-kinin system in bronchoalveolar lavage fluid of patients with severe COVID-19. eBioMedicine, 83, 104195.
+ Open protocol
+ Expand
3

CSF Biomarker Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The collection and storage of CSF used in this study was performed in the same process as follows [22 (link)]. The concentrations of Aβ1–42, t-Tau, and p-Tau181 in CSF were measured using INNOTEST ELISA kit (Fujirebio, Ghent, Belgium) and that of bradykinin (BK) was quantitated with Bradykinin ELISA kit (Enzo Life Sciences, Farmingdale, NY, USA) according to the protocols provided by the manufacturers.
Park J.E., Lim D.S., Cho Y.H., Choi K.Y., Lee J.J., Kim B.C., Lee K.H, & Lee J.S. (2021). Plasma contact factors as novel biomarkers for diagnosing Alzheimer’s disease. Biomarker Research, 9, 5.
+ Open protocol
+ Expand
4

Phospholipid Vesicle Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Small unilamellar phospholipid vesicles (PCPS) composed of 75% (w/w) hen egg L-α-phosphatidylcholine and 25% (w/w) porcine brain L-α-phosphatidylserine (Avanti Polar Lipids) were prepared and quality-controlled as described29 (link). The thrombin S2238 peptidyl substrate was purchased from Chromogenix. Enzygnost Prothrombin Fragment 1.2 micro-ELISA kit was purchased from Siemens. Bradykinin ELISA kit was purchased from Enzo Life Sciences.
Gunaratne R., Kumar S., Frederiksen J.W., Stayrook S., Lohrmann J.L., Perry K., Bompiani K.M., Chabata C.V., Thalji N.K., Ho M.D., Arepally G., Camire R.M., Krishnaswamy S, & Sullenger B.A. (2018). Combination of aptamer and drug for reversible anticoagulation in cardiopulmonary bypass. Nature biotechnology, 36(7), 606-613.
+ Open protocol
+ Expand
5

Quantification of Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols
To evaluate the secretion of IL-6, IL-8 and Angiotensin II, cellular supernatant was collected subsequent to each treatment, aliquoted into eppendorf tubes, and stored at −80°C. IL-6, IL-8 and Angiotensin II concentrations were quantified using commercially available ELISA kits (Human IL-6 Platinum ELISA: BMS213/2, eBioscience, Tasmania, Australia; Human IL-8 Platinum ELISA: BMS204/3, eBioscience; Angiotensin II ELISA kit: ADI-900–204, Enzo Life Sciences, Farmingdale, USA; Bradykinin ELISA kit: ADI-900–206, Enzo Life Sciences) according to procedures recommended by the manufacturer.(5 (link), 26 (link))
Fu Z., Wang F., Liu X., Hu J., Su J., Lu X., Lu A., Cho J.M., Symons J.D., Zou C.J, & Yang T. (2021). Soluble (Pro)Renin Receptor Induces Endothelial Dysfunction and Hypertension in Mice with Diet-Induced Obesity via Activation of Angiotensin II Type 1 Receptor. Clinical science (London, England : 1979), 135(6), 793-810.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!