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4 protocols using iron 2 sulfate

1

Antioxidant and Radical Scavenging Assays

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Chemicals and reagents used such as thiobarbituric acid (TBA), 1,10‐phenanthroline, deoxyribose, gallic acid, Folin–Ciocalteau reagent were procured from Sigma‐Aldrich, Inc., (St. Louis, MO), trichloroacetic acid (TCA) was sourced from Sigma‐Aldrich, Chemie GmbH (Steinheim, Germany), hydrogen peroxide, methanol, sodium nitroprusside, Griess reagent, acetic acid, hydrochloric acid, sodium carbonate, aluminum chloride, potassium acetate, sodium dodecyl sulfate, iron (II) sulfate, potassium ferricyanide, and ferric chloride were sourced from BDH Chemicals Ltd., (Poole, UK). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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2

Enzyme and Antioxidant Assays Protocol

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α‐glucosidase from rats’ intestine, porcine pancreatic α‐amylase, pepsin, p‐nitrophenyl‐α‐D‐glucopyranoside and starch were sourced from (Sigma Chemical Co. St. Louis, MO, USA). 1,10‐phenanthroline, 1,1‐diphenyl–2 picrylhydrazyl (DPPH), Folin–Ciocalteu reagent, deoxyribose, thiobarbituric acid, Tris,3,5‐dinitrosalicylic acid, quercetin, rutin, kaempferol, catechin, epicatechin, gallic, caffeic, and chlorogenic acids were bought from Sigma‐Aldrich, Inc., St Louis, MO. Other chemicals such as aluminum chloride, potassium ferricyanide, sulfuric acid, methanol, hydrogen peroxide, ethanol, Iron (II) sulfate, potassium acetate, sodium dodecyl sulfate (SDS), sodium hydroxide, sodium chloride, potassium chloride, ferric chloride sodium carbonate, sodium acetate, phenol solution, hydrochloric, perchloric, and acetic acids were sourced from (BDH Chemicals Ltd., Poole, UK).
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3

Comprehensive Chemical Reagents Procurement

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Chemical reagents such as acetylthiocholine iodide, sulphanilamide, reduced glutathione, n‐n‐diethyl‐para‐phenylenediamine (DEPPD), ferrous sulfate, semicarbazide were procured from Sigma‐Aldrich Co. (St Louis, Missouri, USA). Trichloroacetic acid (TCA) and sodium acetate was sourced from Sigma‐Aldrich, Chemie GmbH (Steinheim, Germany), hydrogen peroxide, methanol, acetic acid, hydrochloric acid, aluminum chloride, potassium acetate, sodium dodecyl sulfate, iron (II) sulfate, potassium ferrycyanide, and ferric chloride were sourced from BDH Chemicals Ltd. (Poole, England). Ascorbic acid and starch were products of Merck (Darmstadt, Germany). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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4

Antioxidant and Amylase Inhibitory Assays

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Chemical reagents such as Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), 2, 2-diphenyl-1picrylhydrazyl (DPPH), thiobarbituric acid, gallic acid, porcine pancreatic α-amylase, and 1,10-phenanthroline were procured from Sigma Aldrich Co. Trichloroacetic acid was sourced from Sigma Aldrich, Chemie GmbH (Steinheim, Germany). Hydrogen peroxide, methanol, acetic acid, hydrochloric acid, aluminium chloride, potassium acetate, sodium dodecyl sulfate, iron (II) sulfate, potassium ferrycyanide, and ferric chloride were sourced from BDH Chemicals Ltd., (Poole, England, UK). Ascorbic acid and starch were products of Merck (Darmstadt, Germany). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water used was glass distilled.
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