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19 20 edp

Manufactured by Cayman Chemical
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19,20-EDP is a chemical compound used in research and laboratory settings. It is a stable and soluble compound that can be used as a standard or reference material in various analytical techniques. The core function of 19,20-EDP is to provide a reliable and consistent substance for researchers to utilize in their experiments and analyses.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Msppoh, by Cayman Chemical (2 mentions) Matrigel, by Corning (2 mentions) High capacity cdna archive kit, by Thermo Fisher Scientific (1 mentions) 11 12 eet, by Merck Group (1 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Ex 527, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Ampicillin, by GoldBio (1 mentions) Arabinose, by GoldBio (1 mentions) Chloramphenicol, by GoldBio (1 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by GoldBio (1 mentions) 12 13 epome, by Cayman Chemical (1 mentions) Ni nta resin, by GoldBio (1 mentions) δ aminolevulinic acid, by Frontier Scientific (1 mentions) 9 10 epome, by Cayman Chemical (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Annexin 5 fitc and propidium iodide, by BD (1 mentions) Pparδ transcription factor assay kit, by Cayman Chemical (1 mentions)

6 protocols using 19 20 edp

1

Inflammatory Response to EET and EDP

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HRMEC were seeded in 6-well plates and at 85% confluence, cells were placed in serum-reduced culture medium for 12 hours. Cells were treated for 2 hours with vehicle or 1 ng/ml TNFα in the presence or absence of the following treatments, either alone or in combination: 11,12-EET (0.5 μM; Sigma-Aldrich), 19,20-EDP (0.5 μM; Cayman Chemical; Ann Arbor, MI), AUDA (10 μM; Sigma-Aldrich); 11,12-DHET (0.5 μM; Cayman Chemical), or 19,20-DHDP (0.5 μM; Cayman Chemical). Cells were then washed twice with cold PBS, and total RNA was collected using an RNeasy Mini kit (Qiagen, Valencia, CA), according to the manufacturers protocol. Total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Applied Biosystems, Waltham, MA). Quantitative RT-PCR was performed using TaqMan Gene Expression Assays (Applied Biosystems) in duplicate by co-amplification of human VCAM1 and ICAM1 cDNAs compared with 18S as a normalization control.
Capozzi M.E., Hammer S.S., McCollum G.W, & Penn J.S. (2016). Epoxygenated Fatty Acids Inhibit Retinal Vascular Inflammation. Scientific Reports, 6, 39211.
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2

Regulation of LPS-Induced Inflammation

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HL-1 cells were exposed to LPS (1 μg/ml) for 24 h. Where indicated, cells were also treated or co-treated with the following pharmacological agents: 19,20-EDP (1 μM), DHA, metabolic precursor of endogenous EDPs (100 μM), MSPPOH (50 μM) to specifically inhibit CYP epoxygenase activity and block formation of endogenous EDPs from DHA and EX-527 (1 μM) to selectively inhibit SIRT1 activity. Stock solutions of 19,20-EDP, DHA, MSPPOH and EX-527 were prepared in 100% ethanol; final concentrations of both solvents were <0.01% of the treatment solutions. LPS and EX-527 were purchased from Sigma-Aldrich (Oakville, ON, Canada). 19,20-EDP, DHA and MSPPOH were obtained from Cayman Chemical (Ann Arbor, MI, USA).
Samokhvalov V., Jamieson K.L., Vriend J., Quan S, & Seubert J.M. (2015). CYP epoxygenase metabolites of docosahexaenoic acid protect HL-1 cardiac cells against LPS-induced cytotoxicity through SIRT1. Cell Death Discovery, 1, 15054-.
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3

Recombinant Human CYP2J2 Expression

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Human CYP2J2 cDNA was obtained from OriGene (Catalog No. SC321730) and modified as published before.45 (link) Ampicillin, arabinose, chloramphenicol, isopropyl β-D-1-thiogalactopyranoside (IPTG), and Ni-NTA resin were obtained from Gold Biotechnology. δ-aminolevulinic acid was obtained from Frontier Scientific. NADPH and NADP+ were obtained from P212121.com. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L serine (POPS) were purchased from Avanti Polar Lipids, Inc. EPA; DHA; AA; 9,10-EpOMe; 12,13-EpOME; 14,15-EEQ; 17,18-EEQ; 16,17-EDP; and 19,20-EDP were obtained from Cayman Chemical. Restriction enzymes and molecular biology materials were purchased from New England Biolabs. All other materials and reagents used were purchased from Sigma-Aldrich and Fisher Scientific.
Arnold W.R., Baylon J.L., Tajkhorshid E, & Das A. (2016). Asymmetric binding and metabolism of polyunsaturated fatty acids (PUFAs) by CYP2J2 epoxygenase. Biochemistry, 55(50), 6969-6980.
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4

Aortic Sprouting Assay in Mice

Cited 2 times
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Aortae from 4-to-6-week-old C57BL/6J mice were dissected, cut into approximately 1 mm thick rings, embedded in growth factor-reduced Matrigel (354230, Corning, Corning, NY), and then cultured in complete medium (420–500, Cell Systems, Kirkland, WA) supplemented with penicillin-streptomycin (Thermo Fisher) and Culture Boost (Cell Systems). Flunarizine (20 μg/ml) and 19,20-EDP (1 μM, 10175, Cayman, Ann Arbor, MI) were added to the medium 48 h after embedding, and the medium was changed every other day. Images were taken six days after plating. The aortic sprouting areas were quantified using ImageJ with a semi-automated macro plugin for vessel sprout quantification, as previously reported [35 (link),46 (link)].
Gong Y., Tomita Y., Edin M.L., Ren A., Ko M., Yang J., Bull E., Zeldin D.C., Hellström A., Fu Z, & Smith L.E. (2022). Cytochrome P450 oxidase 2J inhibition suppresses choroidal neovascularization in mice. Metabolism: clinical and experimental, 134, 155266.
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5

Lipid Peroxidation and Cell Signaling Assays

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DHA (cat # 90310), N-methylsulphonyl-6-(2-proparglyloxyphenyl) hexanamide (MS-PPOH) (cat # 75770) and 19, 20 – EDP (cat # 10175) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Tissue culture materials were obtained from Invitrogen (Burlingt on, ON, Canada); Bradford protein assay solution from Bio-Rad Laboratories (Mississauga, ON, Canada); Primary and secondary antibodies from Cell Signaling (Pickering, ON, Canada); Trypan blue, MTT, 2′, 7′-Dichlorofluorescin diacetate, myriocin from Sigma–Aldrich (Oakville, ON, Canada). Annexin V-FITC and propidium iodide from BDsciences; Caspase 3 assay kit from Sigma (Saint Louis, MO, USA cat# CASPC3C-1KT); Total 20S proteasome assay kit from Chemicon (EMD Millipore, Etobicoke, ON, Canada cat # APT280); Lipid peroxidation (MDA) cat# ab118970; PPARδ transcription factor assay kit Cayman Chemicals (Ann Arbor, MI, USA cat # 90,310). GSK 3787 cat# 3961; Ceramide cat# 0744 from Tocris bioscience (Bristol, UK). Cell Counting Kit-8 (CCK-8) from Dojindo Molecular Technologies Inc. (Burlington, ON, Canada) cat# CK04–05. EDP methyl esters regioisomer mixtures (1:1:1:1:1:1; 4,5-, 7,8-, 10,11-, 13,14-, 16,17- and 19,20-EDP) were prepared as described previously (Morisseau et al., 2010 (link)).
Samokhvalov V., Zlobine I., Jamieson K.L., Jurasz P., Chen C., Stephen Lee K.S., Hammock B.D, & Seubert J.M. (2014). PPARδ signaling mediates the cytotoxicity of DHA in H9c2 cells. Toxicology letters, 232(1), 10-20.
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6

Aortic Explant Angiogenesis Assay

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Aortae from 3 to 8 week old C57BL/6 J mice were dissected and cut into ~ 1 mm thick rings(Baker et al., 2012 ), embedded into growth factor reduced Matrigel (354230, Corning, Corning, New York), and grown in complete classic medium (4Z0-500, Cell Systems, Kirkland, WA) supplemented with CultureBoost (4CB-500, Cell Systems) and Penicillin-Streptomycin (Thermo Fisher) at 37 °C with 5% CO2. Fenofibric acid (10 μM, 90,568, Sigma-Aldrich), DHA (30 μM, 90310, Cayman Chemical, Ann Arbor, Michigan) or 19,20-EDP (1 μM, 10175, Cayman Chemical) was added to the culture medium 48 h later, and medium was changed every other day. Phase contrast photos of individual explants were taken 6 days after plating and the sprouting area was quantified with ImageJ with a semi-automated macro plugin for quantification of vessel sprouts.
Gong Y., Shao Z., Fu Z., Edin M.L., Sun Y., Liegl R.G., Wang Z., Liu C.H., Burnim S.B., Meng S.S., Lih F.B., SanGiovanni J.P., Zeldin D.C., Hellström A, & Smith L.E. (2016). Fenofibrate Inhibits Cytochrome P450 Epoxygenase 2C Activity to Suppress Pathological Ocular Angiogenesis. EBioMedicine, 13, 201-211.
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