Three HDF strains (Invitrogen, Paisley, UK), passages 3–15, were used throughout. HDFs were maintained at 37 °C in a humidified atmosphere of 5% CO2, as subconfluent monolayers in fibroblast complete medium: Dulbecco's modified Eagle's medium, 0.5% antibiotic solution, and 10% fetal bovine serum (Invitrogen). Sometimes serum-free medium (SFM) was used. All drugs were purchased from Tocris (Bristol, UK), except FGF2 and TGFβ1 (Promocell, Heidelberg, Germany).
Hdf strains
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The HDF strains are a set of laboratory-developed cell lines designed for use in various research applications. They serve as a standardized tool for experiments, providing consistent and reliable cell models. The core function of the HDF strains is to offer researchers a well-characterized and reproducible cell-based system for their studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hdf strains
1
Maintaining Human Dermal Fibroblasts
2
Fibroblast Culture and Maintenance Protocol
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