Bio plex 4

Manufactured by Bio-Rad

Sourced in United States

The Bio-Plex 4.1 software is a data analysis tool designed to work with Bio-Rad's Bio-Plex multiplex assay systems. The software is used to analyze and manage data generated from these multiplex assays, which can simultaneously measure multiple analytes in a single sample.

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Lab products found in correlation

Bio plex 100, by Bio-Rad (4 mentions) Mpxhcyto 60k, by Merck Group (2 mentions) Hscr 32k, by Merck Group (2 mentions) Synergy ht plate reader, by Agilent Technologies (2 mentions) Human leptin elisa kits, by R&D Systems (2 mentions) Milliplex map human cytokine chemokine panel hcytomag 60k, by Merck Group (2 mentions) Bio plex 200 system, by Bio-Rad (1 mentions) Bio plex pro rat cytokine th1 th2 assay, by Bio-Rad (1 mentions) Milliplex map kit, by Merck Group (1 mentions) Dg100, by R&D Systems (1 mentions)

Spelling variants of this product

Bio-Plex (286 citations) Bio-Plex Manager 4.1 software (36 citations) Bio-Plex Manager software version 4.1.1 (33 citations) Bio-Plex Manager Software 4.1 (24 citations) Bio-Plex® Validation Kit 4.0 (2 citations) Bio-Plex Pro Human Cytokine Group I 4-plex (2 citations) Bio-Plex Pro Assay Quick Guide 4 (2 citations)

6 protocols using bio plex 4

Rat Cytokine Expression Profiling

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Expression of 12 rat cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, granulocyte-macrophage colony-stimulating factor [GM-CSF], TNF, and INF-γ) was evaluated from rat plasma samples obtained at baseline, and on D6 and D13 using a commercially available kit (Bio-Plex Pro™ rat cytokine Th1/Th2 assay #171k1002m, Bio-Rad Laboratories) according to the manufacturer’s instructions. Plates were analyzed using Bio-Plex 200 System with Bio-Plex 4.1.1 software (Bio-Rad).

Kyyriäinen J., Kajevu N., Bañuelos I., Lara L., Lipponen A., Balosso S., Hämäläinen E., Das Gupta S., Puhakka N., Natunen T., Ravizza T., Vezzani A., Hiltunen M, & Pitkänen A. (2021). Targeting Oxidative Stress with Antioxidant Duotherapy after Experimental Traumatic Brain Injury. International Journal of Molecular Sciences, 22(19), 10555.

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Multiplex Cytokine Profiling in Serum

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Serum concentrations of 17 different cytokines and immune mediators (Perforin, Granzyme A, Granzyme B, IFN-γ, GMCSF, sFas, sFasL, TNF-α, sCD137, MIP-1α (CCL3), MIP-1β (CCL4), IL-2, IL-4, IL-5, IL-6, IL-10, IL-13) were measured using a multiplex bead array assay from Merck Millipore (Milliplex Map Kit, #HCD8MAG–15 K, Burlington, MA, USA) according to manufacturer’s instructions.
Samples were measured in duplicate on a Luminex LX100 platform (Luminex Corporation Austin, TX, USA). All samples of the same patient were measured on the same plate. The data obtained were collected and analyzed via Bioplex 4.1.1 software (Bio-Rad Laboratories Inc., Hercules, CA, USA). Five-parameter logistic regression (5PL) was used to approximate the standard curves.

Mytilineos D., Ezić J., von Witzleben A., Mytilineos J., Lotfi R., Fürst D., Tsamadou C., Theodoraki M.N., Oster A., Völkel G., Kestler H.A., Brunner C., Schuler P.J., Doescher J., Hoffmann T.K, & Laban S. (2020). Peripheral Cytokine Levels Differ by HPV Status and Change Treatment-Dependently in Patients with Head and Neck Squamous Cell Carcinoma. International Journal of Molecular Sciences, 21(17), 5990.

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Multiplex Assay for Serum Biomarkers

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C-peptide concentrations (ng/ml) were measured using an ELISA (Calbiotech, Spring Valley, CA, REF: CP1795) following the manufacturer’s instructions. IGF-1 was measured by ELISA following the manufacturer’s protocol (R&D Systems, DG100, Minneapolis, MN). Both ELISAs were read using the absorbance function on a Bio-Tek (Winooski, VT) plate reader. Insulin growth factor binding proteins (IGFBP) 1 through 7 were measured by multiplex kit per the manufacturer’s protocol (HIGFBP-53K, Millipore, Billerica, MA). Serum concentrations of the soluble vascular endothelial growth factor receptors (sVEGFR) including sVEGFR-1, sVEGFR-2, and sVEGFR-3, as well as the soluble receptor for advanced glycation end products (sRAGE), and the soluble endothelial growth factor receptor (sEGFR) were determined using a multiplex kit for soluble cytokine receptors (HSCR-32K, Millipore) as per the manufacturer’s instructions. Further, fibroblast growth factor (FGF)-2, endothelial growth factor (EGF), and vascular endothelial growth factor (VEGF) serum concentrations were measured using a multiplex human cytokine/chemokine kit (MPXHCYTO-60K, Millipore). Multiplex assays were analyzed on a Bio-Plex 100 using Bio-Plex 4.1 software (Bio-Rad, Hercules, CA).

Comstock S.S., Xu D., Hortos K., Kovan B., McCaskey S., Pathak D.R, & Fenton J.I. (2014). Association of insulin-related serum factors with colorectal polyp number and type in adult males. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 23(9), 1843-1851.

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Multiplex Cytokine Receptor Assay Protocol

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Serum concentrations of the soluble interleukin-4 receptor (sIL-4R), soluble CD30 (sCD30), soluble gp130 (sgp130), and the soluble interleukin-2 receptor (sIL-2Rα) were determined using a multiplex kit for soluble cytokine receptors (HSCR-32K, Millipore) as per the manufacturer's instructions. Further, IFN-α2, IL-7, IL-8, MCP-3, eotaxin, fms-like tyrosine kinase-3 ligand (FLT-3L), fractalkine, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), GRO1 oncogene (CXCL1/GRO), interferon-gamma (IFN-γ), IL-15, transforming growth factor-alpha (TGF-α), IL-3, IL-4, IL-5, IL-9, IL-13 and MDC serum concentrations were measured using a multiplex human cytokine/chemokine kit (MPXHCYTO-60K, Millipore). Multiplex assays were analyzed on a Bio-Plex 100 using Bio-Plex 4.1 software (Bio-Rad, Hercules, CA).

Comstock S.S., Xu D., Hortos K., Kovan B., McCaskey S., Pathak D.R, & Fenton J.I. (2016). Association of serum cytokines with colorectal polyp number and type in adult males. European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP), 25(3), 173-181.

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Adiponectin, Leptin, and Cytokine Profiling

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Multimeric adiponectin (total, high (HMW), medium (MMW) and low (LMW) molecular weight) was measured by enzyme-linked immunosorbent assay (ELISA) following the manufacturer's instructions (Alpco Diagnostics, Salem, NH). Human leptin ELISA kits were used according to the manufacturer's instructions (R&D Systems, Minneapolis, MN). C-peptide concentrations were determined using a C-peptide ELISA (Calbiotech, Spring Valley, CA) as directed by manufacturer's protocol. The Synergy HT plate reader (Bio-Tek, Winooski, VT) was used to measure ELISA absorbencies. Serum concentrations of IL-6, IL-1α, IL-1β, IL-10, and TNF-α, were determined using a MILLIPLEX™ MAP Human Cytokine/Chemokine panel (HCYTOMAG-60K, Millipore, Billerica, MA) as per the manufacturer's protocol. The soluble receptor for IL-6 (sIL-6R) and the soluble receptor for advanced glycation end products (sRAGE) were analyzed using a MILLIPLEX™ MAP Human Soluble Cytokine Receptor panel (HSCR-32K, Millipore). MILLIPLEX™ samples were analyzed on a Bio-Plex 100 using Bio-Plex 4.1 software (Bio-Rad, Hercules, CA).

Comstock S.S., Lewis M.M., Pathak D.R., Hortos K., Kovan B, & Fenton J.I. (2014). Cross-Sectional Analysis of Obesity and Serum Analytes in Males Identifies sRAGE as a Novel Biomarker Inversely Associated with Diverticulosis. PLoS ONE, 9(4), e95232.

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Multiplex Analysis of Adiponectin and Cytokines

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Multimeric adiponectin (total, high (HMW), medium (MMW) and low (LMW) molecular weight) was measured by ELISA following the manufacturer's instructions (Alpco Diagnostics, Salem, NH). Human leptin ELISA kits were used according to the manufacturer's instructions (R&D Systems, DY398; Minneapolis, MN). The Synergy HT plate reader (Bio-Tek, Winooski, VT) was used to measure ELISA absorbances. Serum was used to assess systemic cytokine concentrations. Serum concentrations of interleukin (IL)-6, tumor-necrosis factor-α (TNF-α), and interferon-γ-inducible protein-10 (IP-10, also known as CXCL10) were determined using a MILLIPLEX™ MAP Human Cytokine/Chemokine panel (HCYTOMAG-60K, Millipore, Billerica, MA) as per the manufacturer's protocol. Samples were analyzed on a Bio-Plex 100 using Bio-Plex 4.1 software (Bio-Rad, Hercules, CA).

Comstock S.S., Hortos K., Kovan B., McCaskey S., Pathak D.R, & Fenton J.I. (2014). Adipokines and Obesity Are Associated with Colorectal Polyps in Adult Males: A Cross-Sectional Study. PLoS ONE, 9(1), e85939.

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