Sch772984

HNSCC cell lines Detroit 562, FaDu, and SCC-9 were obtained from ATCC (Manassas, VA USA).
The following inhibitors from Selleckchem (Houston, TX, USA) were used as follows: foretinib (0.5 µM), MK-2206 (1 µM), S3I-201 (100 µM), SCH772984 (1 µM), and trametinib (25 nM).
Antibodies for PD-L1 (13684), Erk1/2 (9107), P-Erk1/2 (4370), Vinculin (13901), Akt (4691), P-Akt (4060), Stat3 (9139), and P-Stat3 (9145) were obtained from Cell Signaling (Danvers, MA, USA). APC conjugated PD-L1 antibody (17-5983-41), and tubulin-antibody (MS-481-P) were purchased from ThermoFisher Scientific (Waltham, MA, USA), β-Actin antibody (A5316) from Merck, and PE conjugated Met antibody (FAB3582P) from R&D Systems (Minneapolis, MN, USA). All antibodies were applied as stated in the instructions of the manufacturers.

DOX, DRB and TBB were purchased from Sigma (St. Louis, MO, USA). p38 inhibitor SB203580, JNK inhibitor SP600125 and ERK inhibitor SCH772984 were purchased from were purchased from Cell Signaling Technology (Massachusetts, USA), Sigma-Aldrich (St. Louis, USA), Selleck Chemicals (Houston, USA) respectively. Anti-ARC antibody, anti-CK2α antibody, anti-CK2α' antibody and anti-CK2β antibody were obtained from Abcam (Cambridge, UK), anti-PCNA antibody was obtained from Santa Cruz Biotechnology (Texas, USA.), anti-COX IV antibody was obtained from Cell Signaling Technology (Massachusetts, USA). Anti-Phospho-T149 antibody was generated as we described [10 (link)]. Human cervical cancer cells HeLa and human gastric cancer cell line SGC-7901 were as we previously described [9 (link)]. The cells were cultured in Dulbecco's modified Eagle's medium (GIBCO, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin in a humidified atmosphere containing 5% CO₂ at 37°C.