KCMSCs and KGMSCs were collected and washed in washing buffer (1xPBS, 2% FBS and 0.01% Sodium Azide). 50,000 cells were stained with anti CD34 (Becton Dickinson), anti CD90 (BD Bio.), anti-CD105 (Caltag), CD45, (Caltag), anti–CD44 (BD Biosciences) and anti-Flk-1. As control, KCMSCs AND KGMSCS also stained with IgG-FITC, IgG-PE, IgG-APC.After 45 min cells were washed and stained with 7AAD. Cells were analyzed using Beckton Dickinson FACS scan software.
Facs scan software
Manufactured by BD
Sourced in United States
FACS scan software is a computer program designed to analyze and interpret data from flow cytometry instruments. It is used to detect and quantify the characteristics of individual cells or particles passing through a laser beam. The software provides tools for data visualization, analysis, and reporting, allowing users to identify and characterize different cell populations within a sample.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd34, by BD (1 mentions)
Anti cd44, by BD (1 mentions)
Anti cd90, by BD (1 mentions)
Lsrfortessa sorp, by BD (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Solarbio (1 mentions)
Annexin 5 fitc pi apoptosis kit, by Keygen Biotech (1 mentions)
Fc500 flow cytometer, by BD (1 mentions)
Canto 2 flow cytometer, by BD (1 mentions)
4 protocols using facs scan software
1
Characterization of Mesenchymal Stem Cells
2
Apoptotic Podocyte Quantification
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Podocytes were briefly digested with 0.25% trypsin without ethylenediaminetetraacetic acid (EDTA, Solarbio, Beijing, China), and then washed with ice-cold phosphate-buffered saline (PBS). The percentage of apoptotic podocytes was determined using flow cytometry, using the Annexin V-FITC/PI Apoptosis Kit (Keygen Biotech, Nanjing, China), following the manufacturer’s recommendations. Data collection and analysis were performed using a flow cytometer (LSRFortessa™ SORP, Becton, Dickinson and Company, Franklin Lakes, NJ, USA) and FACS scan software (Becton, Dickinson and Company).
3
Apoptosis Quantification in Pancreatic Cells
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Pancreatic cells (5 × 105 cells/mL) were seeded and incubated in 6-well plates for 48 h with the different fatty acids treatments. After that period, the culture medium of each plate (containing cells detached during the cell death process) together with trypsinized cells were centrifuged at 200× g for 5 min after which pellets were resuspended in 100 μL of Annexin–V FLUOS labeling solution (PI and Annexin V–FITC) and incubated 30 min at room temperature in the dark. The percentage of cells undergoing early-stage apoptosis (Annexin V–FITC positive) or late-stage apoptosis/necrosis (Annexin V–FITC and PI positive) was measured with a Becton FC500 flow cytometer. Data were collected using BD FACS scan software, and 10,000 cells were analyzed per sample.
4
Cell Cycle Analysis of L. donovani
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Cell cycle analysis of KalsomeTM10 treated and untreated L.donovani promastigotes was done using flow cytometry. After treatment of promastigotes with varying concentrations of KalsomeTM10 for different time periods, the cells were harvested and washed thrice with PBS and fixed in 50% ethanol (diluted in PBS) for 4 h. The fixed cells were then washed thoroughly, treated with 100 μg/ml RNase A and suspended in 1ml of staining solution. After 1 h, the cells were stained with 40 μg/ml PI for 20 min at room temperature. The percentage of cells in G1, S and G2/M phases of the cell cycle were determined in Becton Dickson Canto II flow cytometer and with BD FACS Scan Software.
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