Atto 655 maleimide

Manufactured by ATTO-TEC

Sourced in Germany

The ATTO 655 maleimide is a fluorescent dye molecule designed for labeling proteins and other biomolecules. It features a maleimide functional group for covalent attachment to sulfhydryl groups (thiols) commonly found in proteins. The ATTO 655 dye has an absorption maximum at 663 nm and an emission maximum at 684 nm, making it suitable for detection in the red region of the visible spectrum.

Automatically generated - may contain errors

Lab products found in correlation

Superdex 200 pg column, by GE Healthcare (1 mentions) Kta pure chromatography system, by GE Healthcare (1 mentions) Cary eclipse, by Agilent Technologies (1 mentions) 5 iodoacetamide fluorescein, by Merck Group (1 mentions) Perfectsil 300 ods c18, by Jasco (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid, by Merck Group (1 mentions) α bromoisobutyryl bromide, by Thermo Fisher Scientific (1 mentions) Copper ii bromide, by Thermo Fisher Scientific (1 mentions) Tin ii 2 ethylhexanoate, by Thermo Fisher Scientific (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions)

4 protocols using atto 655 maleimide

Labeling of KCK-MinD with ATTO 655

Check if the same lab product or an alternative is used in the 5 most similar protocols

Labeling of KCK-MinD with ATTO 655-maleimide
(ATTO-TEC GmbH, Siegen, Germany) was performed according to the manufacturer’s
instruction and with a ratio of three molecules of dye per protein.
Excess dye was removed by gel-filtration chromatography on a 16/600
Superdex 200 pg column (GE Healthcare, Pittsburgh, 492 USA), using
an Äkta Pure chromatography system (GE Healthcare, Pittsburgh,
USA) and storage buffer (50 mM HEPES pH 7.25, 150 mM KCl, 0.1 mM EDTA,
10% glycerol, 0.4 mM TCEP, 0.2 mM ADP) as mobile phase.

Heermann T., Franquelim H.G., Glock P., Harrington L, & Schwille P. (2020). Probing Biomolecular Interactions by a Pattern-Forming Peptide–Conjugate Sensor. Bioconjugate Chemistry, 32(1), 172-181.

+ Open protocol

+ Expand

ATTO655-Labeled IFNAR1 Fluorescence Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

ATTO 655 maleimide was purchased from ATTO-Tec (Siegen, Germany). The EC domains of IFNAR1 WT and SD34 mutant (both including the N349C mutation) were fused to an N-terminal decahistidine tag. The proteins were expressed in Sf9 insect cells using baculovirus infection and purified by immobilized metal-chelating chromatography as previously described. Proteins were labeled with ATTO655 as detailed in reference (Strunk et al., 2008a ). The labeling efficiency was 0.4–0.7 fluorophores per IFNAR1-H10 molecule for the purified proteins. Fluorescence dequenching in solution were carried out with 100 nM of fluorescence-labeled IFNAR1-EC supplemented with 1 mg/ml bovine serum albumin using 120-μl cuvette (Hellma) in Cary Eclipse (Varian) at 20 °C. Labeled proteins were excited at 640 nm and the emission between 660 and 800 nm was recorded.

Li H., Sharma N., General I.J., Schreiber G, & Bahar I. (2017). Dynamic Modulation of Binding Affinity as a Mechanism for Regulating Interferon Signaling. Journal of molecular biology, 429(16), 2571-2589.

+ Open protocol

+ Expand

Solid-Phase Synthesis of Fluorescent C4 Peptide

Check if the same lab product or an alternative is used in the 5 most similar protocols

C4 peptide (RRYCKSTEL) was synthesized on solid phase using standard Fmoc chemistry. For fluorophore labeling, C4 peptide was incubated with a 1.2 molar excess of 5-iodoacetamide-fluorescein (Sigma-Aldrich) or ATTO655-maleimide (ATTO-TEC) in PBS DMF buffer (8.1 mM Na₂HPO₄ pH 6.5, 137 mM NaCl, 2.7 mM KCl, 1.8 mM KH₂PO₄, 20% [v/v] DMF) for 1 hr at 20°C. Peptides were purified by reversed-phase HPLC (Jasco; PerfectSil 300 ODS C₁₈), utilizing a linear acetonitrile gradient from 5% to 80% supplemented with 0.1% (v/v) TFA. Purified fluorescently labeled peptides were snap-frozen in liquid nitrogen and freeze-dried (Lyovac GT2, Heraeus).

Stefan E., Obexer R., Hofmann S., Vu Huu K., Huang Y., Morgner N., Suga H, & Tampé R. (2021). De novo macrocyclic peptides dissect energy coupling of a heterodimeric ABC transporter by multimode allosteric inhibition. eLife, 10, e67732.

+ Open protocol

+ Expand

Surface Functionalization for Biomolecular Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dry toluene, dry dichloromethane (DCM), α-bromoisobutyryl bromide (BIBB), copper(ii)bromide, N,N,N′,N′′,N′′-pentamethyldiethylenetriamine (PMTDA) and tin(ii) 2-ethylhexanoate were purchased from Alfa Aesar. Styrene, ethyl-α-bromoisobutyrate (EBIB), triethylamine (TEA) dimethylformamide (DMF) and aminopropyltrimethoxysilane (APTMS) were obtained from Sigma Aldrich. N-Aminoethyl-aza-2,2-dimethyl-4-methylsilacyclopentane (thereafter referred to as cycloazasilane) and 2,2-dimethoxy-1-thia-2-silacyclopentane (thereafter referred to as cyclothiasilane) were purchased from Gelest. The fluorescent dyes ATTO 647 NHS and ATTO 655 maleimide were obtained from ATTO-TEC GmbH, Germany. Dibenzocyclooctyne-(polyethyleneglycol-12)-N-hydroxysuccinimidyl ester (NHS-PEG12-DBCO) was purchased from Click Chemistry Tools, USA. Azido-HaloTag-Ligand (click-HTL) was synthesized as described previously.^{31 (link)} The IUPAC name of click-HTL is 3-(4-azidophenyl)-N-(2-(2-(6-chlorohexyloxy)ethoxy)ethyl)propanamide. HaloTag-fused monomeric enhanced GFP protein (HaloTag-mEGFP) and poly(l-lysine)-graft-poly(ethylene glycol) terminated with methoxy groups (PLL-PEG-OMe) were prepared according protocols described previously.^{32,33 (link)} Chemicals for preparing HEPES buffered saline (HBS), such as 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid and NaCl were purchased from Sigma Aldrich.

Philippi M., You C., Richter C.P., Schmidt M., Thien J., Liße D., Wollschläger J., Piehler J, & Steinhart M. (2019). Close-packed silane nanodot arrays by capillary nanostamping coupled with heterocyclic silane ring opening. RSC Advances, 9(43), 24742-24750.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!