C96 gal4

Manufactured by BD

Sourced in Austria, Morocco

The C96-Gal4 is a lab equipment product designed for genetic manipulation experiments. It serves as a driver for the expression of target genes in model organisms. The core function of the C96-Gal4 is to enable the controlled and targeted expression of desired genes in research subjects.

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Lab products found in correlation

Uas bskdn, by BD (1 mentions) Gmr gal4, by BD (1 mentions) Apgal4, by BD (1 mentions) Dpp gal4, by BD (1 mentions) En gal4, by BD (1 mentions) Ptc gal4, by BD (1 mentions)

2 protocols using c96 gal4

Drosophila Genetics for Developmental Studies

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All Drosophila strains were grown and maintained at room temperature (22 °C). Klp64D^k1 allele causes a premature stop codon¹⁰ (stock number 5578, Bloomington Drosophila Stock center (BDSC), USA). UAS lines used were UAS-Klp64D RNAi (10642R-1 and 10642R-2 from National Institute of Genetics, Japan, v45373 from Vienna Drosophila Resource Center, Austria), UAS-Klp64D^{33 (link)}, UAS-arm RNAi (8515 BDSC), UAS-arm^S10^{34 (link)}, UAS-arm, arm² mutant (8369, 8554 BDSC) and C96-Gal4 (BDSC).

Vuong L.T., Won J.H., Nguyen M.B, & Choi K.W. (2020). Role of Armadillo repeat 2 and kinesin-II motor subunit Klp64D for wingless signaling in Drosophila. Scientific Reports, 10, 13864.

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Drosophila Genetics and Imaging Protocols

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All fly stocks were maintained on standard cornmeal/yeast/molasses/agar medium at 25 °C as per standard procedures. Oregon-R flies were used as wild-type controls. UAS-GFP-zip, UAS-GFP-Zip DN (UAS-Myo II-Neck-Rod), UAS-Myo II-Rod, and UAS-Myo II-Rod (delta N_term58) [26 (link)] were obtained as a gift from Prof. Daniel P. Kiehart (Department of Biology, Duke University, Durham, NC). UAS-Notch-FL [27 (link)], UAS-Notch-ICD, UAS-Notch-DN [28 (link)], and Notch pathway components were kindly provided by Prof. S. Artavanis-Tsakonas (Department of Cell Biology, Harvard Medical School, Boston, MA).

cn¹ bw¹ sp¹ zip¹/CyO (BDSC 4199),

P{FRT(w^hs)}G13 zip²/CyO (BDSC 8739),

P{lacZ.w⁺}276, y¹ sc* v¹ sev²¹; P{TRiP.GL00623}attP40 (BDSC 37480), y¹ sc* v¹ sev²¹; P{TRiP.HMS01618}attP2 (BDSC 65947),

Sqh^AX3-GFP (BDSC 57144), UAS-Bsk-DN (BDSC 6409), vg-GAL4 (BDSC 8222), GMR-GAL4 (BDSC 8121), ptc-GAL4 (BDSC 2017), en-GAL4 (BDSC 30564), ap-GAL4 (BDSC 56807), dpp-GAL4 (BDSC 1553), and C96-GAL4 (BDSC 43343) stocks were obtained from Bloomington stock center. All crosses were performed at 25 °C. The combination lines vg-GAL4/UAS-GFP-zip and UAS-Notch-DN/C96-GAL4 were made with the help of appropriate genetic crosses.

Verma D., Singh A., Singh J., Mutsuddi M, & Mukherjee A. (2024). Regulation of Notch signaling by non-muscle myosin II Zipper in Drosophila. Cellular and Molecular Life Sciences: CMLS, 81(1), 195.

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