Bone marrow cells, splenocytes, and peritoneal wash cells were depleted of red blood cells and stained with various combinations of the following antibodies. Bone marrow: anti-CD43 FITC (BD Biosciences), anti-IgM PE (BD Biosciences), anti-B220 PerCP-Cy5.5 (Tonbo Biosciences), and anti-CD93 APC (Invitrogen). Spleen: anti-CD21 FITC (BD Biosciences), anti-CD23 PE (BD Biosciences), anti-CD95 PE (BD Biosciences), anti-IgM PErCP-Cy5.5 (BD Biosciences), B220 PerCP-Cy5.5, anti-B220 APC (Tonbo Biosciences), or anti-GL7 APC (BD Biosciences). Peritoneal wash: anti-CD11b FITC (BD Biosciences), anti-CD5 PE (Tonbo Biosciences), anti-IgM PErCP-Cy5.5, anti-B220 APC. Cultured B cells were stained with anti-CD138 PE (BD Biosciences), anti-B220 PerCP-Cy5.5, and anti-IgG1 biotin (BD Biosciences) plus streptavidin APC (Tonbo Biosciences). Samples were run on a FACS Calibur (BD) and analyzed with Flowjo (Treestar).
Anti cd43 fitc
Manufactured by BD
Anti-CD43 FITC is a fluorescently-labeled monoclonal antibody that binds to the CD43 surface antigen. CD43 is a sialoglycoprotein expressed on the surface of most hematopoietic cells, including T cells, B cells, and monocytes.
Automatically generated - may contain errors
Lab products found in correlation
Anti igm pe, by BD (1 mentions)
Anti cd21 fitc, by BD (1 mentions)
Anti cd23 pe, by BD (1 mentions)
Anti cd95 pe, by BD (1 mentions)
B220 percp cy5, by Cytek Biosciences (1 mentions)
Anti b220 apc, by Cytek Biosciences (1 mentions)
Anti gl7 apc, by BD (1 mentions)
Pe anti cd138, by BD (1 mentions)
Anti b220 percp cy5, by BD (1 mentions)
Anti igg1 biotin, by BD (1 mentions)
Streptavidin apc, by Cytek Biosciences (1 mentions)
Anti igm per cp cy5, by BD (1 mentions)
Anti cd11b fitc, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Anti b220 pe cy7, by BD (1 mentions)
Facsaria 2 cell sorter, by BD (1 mentions)
Lsrfortessa, by BD (1 mentions)
Anti cd31 pe, by BD (1 mentions)
Anti cd73 pe, by BD (1 mentions)
Anti cd31 fitc, by BD (1 mentions)
4 protocols using anti cd43 fitc
1
Flow Cytometric Immunophenotyping of Murine Immune Cells
2
Multicolor Flow Cytometry of Embryonic Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Multicolour flow cytometry analysis of embryonic tissues was performed using LSR Fortessa (BD). Cell populations were isolated using a FACSAria II cell sorter (BD). The following anti-mouse antibodies (all from BD Pharmingen) were used for staining: anti-CD45 BD V500 or BD V450 (clone 30-F11); anti-CD5 BD V450 or PE clone (53-7.3); anti-CD43 FITC (clone S7); anti-B220-PE-cy7 (clone RA3-6B2); anti-CD3e-APC (clone 145-2C11); anti-CD19-PE or PerCP-cy5.5 (clone 1D3); anti-Ter119-V500 or PerCPcy5.5. Gating strategy was defined based on fluorescence minus one (FMO) control staining where one of each antibody was substituted by isotype control (IC) conjugated with corresponding fluorochrome. All analyses and sorting were done on the basis of 7AAD dead cell and Ter119+ cell exclusion.
3
Multiparametric Flow Cytometry of Differentiated EBs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells from dissociated EB were filtered through 50 μm filter (Partec) before stained for FACS analysis. All antibodies were purchased from BD Bioscience, including anti-CD34-APC (#555824), anti-CD43−FITC (#555475), anti-CD31-PE (#555446), anti-CD31-FITC (#555445), anti-CD73-PE (#550257), anti-CD184- PE-Cy7 (#560669), and anti-CD309- Alexa Flour 647 (#560495). Cells were stained for 30 minutes at 4 degree in the dark then washed twice with PBS supplemented with 2% FBS before flow cytometry. DAPI was used for selection of live cells. All antibodies for flow cytometry were diluted at 1:5 ratio.
4
Dissecting IL-10 Producing B Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 4 h of stimulation, PBMCs were stained with a combination of the following antibodies from BD Bioscience: anti-CD19-PerCP (cat #345–778), anti-CD43-FITC (cat #555–475), anti-CD27-PECy7 (cat #560–609), anti-CD24-FITC (cat #555–427), anti-CD38-PECy7 (cat #335–825), anti-CD25 FITC (cat #555–431), anti-IL-10 APC (cat #554–707) or anti-IL-10-PE (cat #559–330). Anti-CD5 APC was from Dako (cat #C7242, Glostrup, Denmark) and anti-TIM-1 PE was purchased from Biolegend (cat #353–904).
After 48 h of stimulation, IL-10 was detected with the following BD Bioscience Abs: anti-CD19 APC (cat #555–415), anti-CD14 FITC (cat #555–397), anti-CD4 PerCP (cat #345–770), anti-CD8 PECy7 (cat #557–746) and anti-IL-10 PE (cat #559–330). Live/Dead Fixable Near InfraRed staining (cat #L10119; Molecular Probes, Invitrogen, Carlsbad, CA) was included.
The cells were acquired with a FACS Canto (BD Bioscience) flow cytometer with argon laser (488 nm) and Helium-Neon laser (633nm) excitation.
All analyses were carried out using FlowJo V10 (TreeStar, Ashland, OR). Dead cells were excluded based on Live/Dead Fixable Near InfraRed staining and B cells were identified as CD19+ events within a morphological lymphocyte gate. Individual IL-10+ B cells were identified using the gating strategy demonstrated inS1 Fig .
After 48 h of stimulation, IL-10 was detected with the following BD Bioscience Abs: anti-CD19 APC (cat #555–415), anti-CD14 FITC (cat #555–397), anti-CD4 PerCP (cat #345–770), anti-CD8 PECy7 (cat #557–746) and anti-IL-10 PE (cat #559–330). Live/Dead Fixable Near InfraRed staining (cat #L10119; Molecular Probes, Invitrogen, Carlsbad, CA) was included.
The cells were acquired with a FACS Canto (BD Bioscience) flow cytometer with argon laser (488 nm) and Helium-Neon laser (633nm) excitation.
All analyses were carried out using FlowJo V10 (TreeStar, Ashland, OR). Dead cells were excluded based on Live/Dead Fixable Near InfraRed staining and B cells were identified as CD19+ events within a morphological lymphocyte gate. Individual IL-10+ B cells were identified using the gating strategy demonstrated in
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!