Peptides were desalted using BioPureSPN C18 MINI spin columns (The Nest Group) according to the manufacturer’s instructions and dried down using a SpeedVac system. Peptides were resuspended in LC solvent A (1% acetonitrile, 0.1% formic acid (FA)) and spiked with iRT kit calibration peptides (Biognosys). Peptide concentrations were determined using a UV/Vis Spectrometer at 280 nm (SPECTROstar Nano, BMG Labtech).
Irt kit calibration peptides
Manufactured by Biognosys
Sourced in Switzerland
The IRT kit calibration peptides are a set of synthetic peptides designed for the calibration of liquid chromatography-mass spectrometry (LC-MS) systems used in targeted proteomics experiments. These peptides provide a standardized reference for the retention time (RT) and ion mobility (IM) values, which are critical parameters in the identification and quantification of proteins in complex biological samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using irt kit calibration peptides
1
Peptide Desalting and Quantification
2
Peptide Desalting and Resuspension
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Peptides were desalted using C18 96-well MACROSpinplates (The Nest Group, Southborough, MA, USA) according to the manufacturer’s instructions and dried down using a SpeedVac system. Peptides were resuspended in 40 μl LC solvent A (1% acetonitrile, 0.1% formic acid (FA)) and spiked with iRTkit calibration peptides (Biognosys, Schlieren, Switzerland) prior to mass spectrometric analyses.
3
Peptide Desalting and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peptides were desalted using BioPureSPN C18 MINI spin columns (The Nest Group) according to the manufacturer's instructions and dried down using a SpeedVac system. Peptides were resuspended in LC solvent A (1% acetonitrile, 0.1% formic acid (FA)) and spiked with iRT kit calibration peptides (Biognosys).
Peptide concentrations were determined using a UV/VIS Spectrometer at 280nm (SPECTROstar Nano, BMG Labtech).
Peptide concentrations were determined using a UV/VIS Spectrometer at 280nm (SPECTROstar Nano, BMG Labtech).
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