B220 alexfluor700

Manufactured by BD

The B220-AlexFluor700 is a lab equipment product designed for flow cytometry applications. It is a fluorescently-labeled antibody that binds to the B220 cell surface antigen, which is expressed on B lymphocytes. The AlexaFluor700 fluorescent dye is used to label the antibody, allowing for detection and quantification of B220-positive cells by flow cytometry.

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Lab products found in correlation

Facsverse system, by BD (1 mentions) Ki67 apc, by BD (1 mentions) B220 pb, by BD (1 mentions) B220 fitc, by BD (1 mentions) Cd4 pb, by BD (1 mentions) B220 percp, by BD (1 mentions) Facsuite software, by BD (1 mentions) Cd8 apc, by BD (1 mentions)

1 protocol using b220 alexfluor700

Immunophenotyping of NZB/W F1 Mice

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For assessment of T and B cell populations in NZB/W F1 mice, splenocytes and/or bone marrow were harvested following drug treatment and analyzed by flow cytometry. Single immune cell suspensions were prepared from spleen or bone marrow after red blood cell lysis. Cells were incubated with anti-CD16/CD32 (Fc block, clone 2.4G2) and stained with various combinations of antibodies. Antibodies used for these analyses were murine-reactive CD21-FITC, B220-FITC, CD3-PerCPCy5.5, CD11b-PerCPCy5.5, Gr1-PerCPCy5.5, B220- PerCP, CD23-PECy7, B220-AlexFluor700, IgD-BV510, CD38- FITC, CD44-FITC, B220-FITC, CD69-PE, CD86-PE, CD138-PE, CD3- PerCPCy5.5, CD5-PerCPCy5.5, B220-PerCP, ICOS-PECy7, CD4-PB, B220-PB, B220-AlexFluor700, IgD-BV510, CD8-APC, Ki67-APC (all antibodies and Fc block from BD Biosciences). Flow cytometry was run on BD FACSVerse™ System with BD FACSuite™ Software for acquisition, and data analysis was done using BD FlowJo™ Software.

Muchamuel T., Fan R.A., Anderl J.L., Bomba D.J., Johnson H.W., Lowe E., Tuch B.B., McMinn D.L., Millare B, & Kirk C.J. (2023). Zetomipzomib (KZR-616) attenuates lupus in mice via modulation of innate and adaptive immune responses. Frontiers in Immunology, 14, 1043680.

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