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Eclipse ni2

Manufactured by Nikon
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The Eclipse Ni2 is a high-performance upright microscope system designed for a wide range of advanced microscopy applications. It features a sturdy and ergonomic design, with a focus on optical performance, flexibility, and user-friendliness.

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Lab products found in correlation

Axio imager z2 microscope, by Zeiss (2 mentions) Nis element, by Nikon (2 mentions) Zen 3, by Zeiss (2 mentions) Apotome attachment, by Zeiss (2 mentions)

2 protocols using eclipse ni2

1

Quantitative Fluorescence Microscopy Analysis

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Images of immunofluorescence were captured using an Axio-imager Z2 microscope fitted with an apotome attachment for structured illumination (Carl Zeiss MicroImaging) and a Nikon Eclipse Ni2. 20X (0.8 NA), 40X (0.95 NA), and 63X (1.4 NA) objectives were used. Images were taken using Zen 3.2 (Zeiss) or NIS-Elements (Nikon). For measurements of AIS streptavidin fluorescence intensity, 20 neurons per timepoint per replicate were imaged and line scans were drawn using Zen 3.2 software. AIS were identified by immunostaining using known AIS proteins (AnkG, β4 spectrin, Nfasc, or NrCAM). For the analysis of cerebellar pinceau, a region of interest including the Purkinje neuron AIS (labeled by Nfasc) was manually drawn. The fluorescence intensity for Kv1.2 was measured for each region of interest and normalized to the area. All measurements were taken with the same exposure times and immunolabeling was also performed at the same time. Images were exported to Fiji, Adobe Photoshop, and Adobe illustrator for figure presentation. Some figures were generated using Biorender.
Ogawa Y., Lim B.C., George S., Oses-Prieto J.A., Rasband J.M., Eshed-Eisenbach Y., Hamdan H., Nair S., Boato F., Peles E., Burlingame A.L., Van Aelst L, & Rasband M.N. (2023). Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments. Nature Communications, 14, 6797.
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2

Immunofluorescence Microscopy Protocol

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Images of immunofluorescence were captured using an Axio-imager Z2 microscope fitted with an apotome attachment for structured illumination (Carl Zeiss MicroImaging) and a Nikon Eclipse Ni2. 20X (0.8 NA), 40X (0.95 NA), and 63X (1.4 NA) objectives were used. Images were taken using Zen 3.2 (Zeiss) or NIS-Elements (Nikon). For measurements of AIS streptavidin fluorescence intensity, 20 neurons per timepoint per replicate were imaged and line scans were drawn using Zen 3.2 software. Images were exported to Fiji, Adobe Photoshop, and Adobe illustrator for figure presentation. Some figures were generated using Biorender.
Ogawa Y., Lim B.C., George S., Oses-Prieto J.A., Rasband J.M., Eshed-Eisenbach Y., Nair S., Boato F., Peles E., Burlingame A.L., Van Aelst L, & Rasband M.N. (2023). Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments. bioRxiv.
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