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White bottomed assay plate

Manufactured by Corning
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The White-bottomed assay plate is a laboratory equipment piece designed for use in various assay procedures. It provides a consistent and reliable platform for conducting experiments and measurements. The plate features a white bottom that can enhance the visibility and contrast of assay results, enabling more accurate data collection.

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Lab products found in correlation

Phenol red free high glucose dmem, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions) Dual luciferase reporter kit, by Promega (2 mentions) Spark m10, by Tecan (2 mentions)

2 protocols using white bottomed assay plate

1

Quantifying Hedgehog Signaling Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Shh-LIGHT2 cells were seeded in 96-well plates at a density of 3.5 × 104 cells/well. After 24 h, growth media was replaced with serial dilutions of 2, 20(S)-OHC, 20(R)-OHC, or SAG (CAS no. 912545-86-9, Carbosynth, FS76762) in low-serum media (phenol red-free high-glucose DMEM, Gibco, 21063045) containing 0.5% bovine calf serum (CS, ATCC, CRL-1658), 100 U/mL of penicillin-streptomycin (Gibco, 15140163), and 1 mM sodium pyruvate) at a normalized DMSO concentration of 0.2%. After 30 h, cells were washed with PBS and treated with Passive Lysis Buffer (20 μL/well, Promega, E1941) at room temperature for 15 min with rocking. 10 μL lysate from each well was transferred to a white-bottomed assay plate (Corning, 3912) for Firefly and Renilla luciferase measurements using a Dual Luciferase Reporter kit (Promega, E1960) on a Tecan Spark M10 multimode plate reader. Gli activity was calculated as the ratio of Firefly/Renilla luciferase signal and percent Gli activation was assessed relative to DMSO-only control values. Dose-response curves were generated using GraphPad Prism software.
Cheng Y.S., Zhang T., Ma X., Pratuangtham S., Zhang G.C., Ondrus A.A., Mafi A., Lomenick B., Jones J.J, & Ondrus A.E. (2021). A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. Nature chemical biology, 17(12), 1271-1280.
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2

Quantifying Hedgehog Signaling Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Shh-LIGHT2 cells were seeded in 96-well plates at a density of 3.5 × 104 cells/well. After 24 h, growth media was replaced with serial dilutions of 2, 20(S)-OHC, 20(R)-OHC, or SAG (CAS no. 912545-86-9, Carbosynth, FS76762) in low-serum media (phenol red-free high-glucose DMEM, Gibco, 21063045) containing 0.5% bovine calf serum (CS, ATCC, CRL-1658), 100 U/mL of penicillin-streptomycin (Gibco, 15140163), and 1 mM sodium pyruvate) at a normalized DMSO concentration of 0.2%. After 30 h, cells were washed with PBS and treated with Passive Lysis Buffer (20 μL/well, Promega, E1941) at room temperature for 15 min with rocking. 10 μL lysate from each well was transferred to a white-bottomed assay plate (Corning, 3912) for Firefly and Renilla luciferase measurements using a Dual Luciferase Reporter kit (Promega, E1960) on a Tecan Spark M10 multimode plate reader. Gli activity was calculated as the ratio of Firefly/Renilla luciferase signal and percent Gli activation was assessed relative to DMSO-only control values. Dose-response curves were generated using GraphPad Prism software.
Cheng Y.S., Zhang T., Ma X., Pratuangtham S., Zhang G.C., Ondrus A.A., Mafi A., Lomenick B., Jones J.J, & Ondrus A.E. (2021). A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. Nature chemical biology, 17(12), 1271-1280.
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