Propidium iodide double staining

Manufactured by Merck Group

Propidium iodide double staining is a laboratory technique used to assess cell viability and cell cycle status. It involves the use of two fluorescent dyes, propidium iodide and a cell-permeable dye, to stain the DNA content of cells. This method allows for the identification of live, dead, and apoptotic cells within a sample.

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Lab products found in correlation

Imager z1 microscope, by Zeiss (2 mentions) Apo one homogeneous caspase 3 7 assay kit, by Promega (2 mentions) Annexin 5, by Merck Group (2 mentions) Cisplatin, by Merck Group (1 mentions) Adriamycin adr, by Merck Group (1 mentions) Camptothecin cpt, by Merck Group (1 mentions)

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Propidium iodide (7211 citations) Propidium iodide staining (38 citations)

2 protocols using propidium iodide double staining

Apoptosis Assays in Oral Cancer Cells

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Cal-27 and Scc-9 cells were treated with the IC₅₀ of cisplatin(Sigma, USA) for 24 hours for an apoptosis assay [37 (link), 58 (link)]. Additionally, Cal-27 and Scc-9 cells were treated with 2×10⁻⁶ M adriamycin(ADR) (Sigma, USA) or 15×10⁻⁶ M camptothecin(CPT) (Sigma, USA) for 24 hours for an apoptosis assay. TUNEL technology was performed using a kit from Roche (Cat.No.11684795910). The detection procedures were in accordance with the kit instructions. Sections were examined with an ImagerZ1 microscope (Zeiss, Jena, Germany). An investigator blind to the treatment quantified 20 random fields of samples. Caspase-3/7 activity was determined using an Apo-ONE® Homogeneous Caspase-3/7 assay kit from Promega according to the manufacturer's protocol. Flow cytometry was performed using Annexin V and propidium iodide double staining (Sigma-Aldrich).

Fan S., Liu B., Sun L., Lv X.B., Lin Z., Chen W., Chen W., Tang Q., Wang Y., Su Y., Jin S., Zhang D., Zhong J., Li Y., Wen B., Zhang Z., Yang P., Zhou B., Liang Q., Yu X., Zhu Y., Hu P., Chu J., Huang W., Feng Y., Peng H., Huang Q., Song E, & Li J. (2015). Mitochondrial fission determines cisplatin sensitivity in tongue squamous cell carcinoma through the BRCA1–miR-593-5p–MFF axis. Oncotarget, 6(17), 14885-14904.

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Apoptosis Detection Methods Comparison

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Apoptosis was detected using TUNEL, flow cytometry and caspase-3/7 activity assays (7 (link)). TUNEL assays were performed using a kit from Roche (Cat. No. 11684795910) according to the user’s instructions. Sections were examined with an ImagerZ1 microscope (Zeiss, Jena, Germany). An investigator blinded to the treatment quantified 20 random fields of samples. Flow cytometry was performed using Annexin V and propidium iodide double staining (Sigma-Aldrich). Caspase-3/7 activity was determined using an Apo-ONE® Homogeneous Caspase-3/7 assay kit from Promega according to the manufacturer’s protocol.

Tian T., Lv X., Pan G., Lu Y., Chen W., He W., Lei X., Zhang H., Liu M., Sun S., Ou Z., Lin X., Cai L., He L., Tu Z., Wang X., Tannous B.A., Ferrone S., Li J, & Fan S. (2019). Long noncoding RNA MPRL promotes mitochondrial fission and cisplatin chemosensitivity via disruption of pre-miRNA processing. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(12), 3673-3688.

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