Mice were i.v. inoculated with 1x106 EL4-CD19+ cells on day 0 and i.v. treated with 9x106 vexGFP-CAR T cells or 2.5x106 Thy1.1+ CAR T cells the following day as the experiment indicated. Spleen and bone marrow cells were harvested, lysed with ammonium-chloride-potassium (ACK) red blood cell lysing buffer (Lonza) and resuspended in media for subsequent cell counting, phenotypical, and functional analysis.
Ack red blood cell lysing buffer
Manufactured by Lonza
ACK red blood cell lysing buffer is a reagent used to lyse or break down red blood cells in biological samples. It functions by disrupting the cell membranes of red blood cells, allowing for the isolation and analysis of other cell types, such as lymphocytes or leukocytes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ack red blood cell lysing buffer
1
Mouse Model of Adoptive CAR T-cell Therapy
2
Mouse Model of Adoptive CAR T-cell Therapy
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