Allprep ffpe dna extraction kit

DNA from FFPE samples was extracted using the AllPrep-FFPE-DNA Extraction Kit (Qiagen). Library preparation for each sample was performed according to the instructions provided by the manufacturer. Briefly, 3 μg of high-quality genomic DNA was randomly cut into 150 to 200-bp fragments. Agencourt AMPure XP beads (Agencourt Biosciences, Beverly, Massachusetts, USA) were used for size selection, and PCR amplification was then performed. The whole-exome sequences were enriched using the SureSelect Human All Exon Kit V5 (Agilent) and pooled and sequenced using the Illumina HiSeq Xten platform.

Genomic DNA was extracted from FFPE samples using an AllPrep FFPE DNA Extraction Kit (Qiagen, Hildon, Germany) according to the manufacturer’s instructions. BCR complementarity determining region 3 (CDR3) was amplified using a Multiplex PCR kit (Qiagen, Hilden, Germany), and a 100 to 200-bp fragment was selected and purified using a QIAquick gel purification kit (Qiagen, Hilden, Germany). The DNA was then subjected to end repair. The A tail was added, ligated, adapte, subsequently amplified by PCR and further purified with Agencourt AMPure XP beads (Agencourt Biosciences, Beverly, MA, USA). The sequencing library was quantified with Invitrogen Quibit 2.0 (Invitrogen, California, USA).