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Anti phosphor pi3k

Manufactured by Cell Signaling Technology
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Anti-phospho-PI3K is a laboratory reagent used to detect and quantify the phosphorylation of Phosphoinositide 3-kinase (PI3K), a key enzyme involved in various cellular signaling pathways. This product provides a tool for researchers to study the activation and regulation of PI3K in biological systems.

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Lab products found in correlation

Nsc228155, by MedChemExpress (1 mentions) Xba 1, by Toyobo (1 mentions) Kod fx neo, by Toyobo (1 mentions) Lysis buffer, by Cell Signaling Technology (1 mentions) Polyvinylidene difluoride pvdf membrane, by GE Healthcare (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Fibrinogen alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg horseradish peroxidase conjugate hrp, by Cell Signaling Technology (1 mentions) Enhanced chemiluminesence solution ecl, by GE Healthcare (1 mentions) Anti vasp, by Cell Signaling Technology (1 mentions) Anti pi3k, by Cell Signaling Technology (1 mentions) Anti phosphor akt, by Cell Signaling Technology (1 mentions)

2 protocols using anti phosphor pi3k

1

Molecular Signaling Pathway Regulation

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Regarding the primary antibodies used in the study, anti-GAPDH (60004), anti-MYC(10828-1-AP), anti-LPCAT1 (66044, Mouse Monoclonal), and anti-SREBP1 (14088) were purchased from Proteintech (IL, USA); anti-MSMO1 (Thermo Fisher, USA), anti-SQLE, anti-LPCAT1 (ab214034, Rabbit Monoclonal), and anti-INSIG1 (ab70784, Rabbit polyclonal) were purchased from Abcam (Cambridge, UK); anti-phospho-SREBP1 (AF3283) was purchased from Affinity (OH, USA); anti-EGFR (#4267S), anti-phosphor-EGFR (#3777S), anti-PI3K (#4228), and anti-phosphor-PI3K (#4292) were purchased from Cell Signaling Technology (Danvers, PA USA). The restriction endonucleases (KpnI, Hind III, Nhe I, Xba I), KOD FX Neo were purchased from TOYOBO (SHANGHAI) BIOTECH CO., LTD. The Seamless Assembly Cloning Kit (CloneSmarter, USA) was purchased from Taihe Biotechnology Co., LTD. The activators 740 Y-P and NSC 228155 were purchased from MedChemExpress (NJ, USA).
Tao M., Luo J., Gu T., Yu X., Song Z., Jun Y., Gu H., Han K., Huang X., Yu W., Sun S., Zhang Z., Liu L., Chen X., Zhang L., Luo C, & Wang Q. (2021). LPCAT1 reprogramming cholesterol metabolism promotes the progression of esophageal squamous cell carcinoma. Cell Death & Disease, 12(9), 845.
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2

Collagen-Mediated Platelet Activation Signaling

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Collagen was purchased from Chrono-Log Co. (Havertown, PA., USA). ATP assay kit was purchased from Biomedical Research Service Center (Buffalo, NY., USA). Cordycepin, A-kinase inhibitor Rp-8-Br-cAMPS, cGMP-dependent protein kinase (G-kinase) inhibitor Rp-8-Br-cGMPS, A-kinase activator pCPT-cAMP, and G-kinase activator 8-Br-cGMP were obtained from Sigma Chemical Corporation (St. Louis, MO., USA). Serotonin ELISA kit was purchased from Labor Diagnostika Nord GmbH & CO. (Nordhorn, Germany). Anti-VASP, anti-phosphor- VASP (Ser157), anti-phosphor-VASP (Ser239), anti-PI3K, anti-phosphor- PI3K, anti-Akt, anti-phosphor-Akt, and anti-rabbit IgG-horseradish peroxidase conjugate (HRP), and lysis buffer were obtained from Cell Signaling (Beverly, MA., USA). Poly-vinylidene difluoride (PVDF) membrane was from GE Healthcare (Piseataway, NJ., USA). Enhanced chemiluminesence solution (ECL) was from GE Healthcare (Chalfont St., Giles, Buckinghamshire, UK). Fibrinogen Alexa Fluor 488 conjugate was obtained from Invitrogen Molecular Probes (Eugene, OR., USA).
Lee D.H., Kim H.H., Lim D.H., Kim J.L, & Park H.J. (2015). Effect of Cordycepin-Enriched WIB801C from Cordyceps militaris Suppressing Fibrinogen Binding to Glycoprotein IIb/IIIa. Biomolecules & Therapeutics, 23(1), 60-70.
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