Dox HCl and Span20 were purchased from Sigma Aldrich®, St. Louis, MO, USA. The synthesis of plier-like cationic lipid B (PCL-B) was kindly assisted by Ramkhamhaeng University and Mahasarakham University, Thailand. Cholesterol (Chol) was acquired from Carlo Erba Reagent (Milan, Italy). siAF488 was obtained from Qiagen (Santa Clarita, CA, USA). siRNA targeting apoptosis-related proteins including Mcl-1, Bcl-2, and survivin was obtained from Ambion™ Silencer™ siRNA, Thermo Fisher Scientific (Waltham, MA, USA). Lipo2k and siNT were purchased from Invitrogen (Carlsbad, CA, USA).
Lipo2k
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Lipo2k is a precision laboratory instrument designed for lipoprotein analysis. It utilizes advanced techniques to accurately measure and characterize lipoproteins within a sample. The core function of the Lipo2k is to provide researchers and scientists with detailed lipoprotein data to support their scientific investigations.
Automatically generated - may contain errors
2 protocols using lipo2k
1
Cationic Lipid-Mediated siRNA Delivery
2
Transfection of A549 cells using Lipo2k and BPEI
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The GFP-encoding plasmid DNA (pCMV-EGFP) (Clontech Laboratories Inc., Palo Alto, CA, USA) complex with Lipo2k (Invitrogen) was prepared according to the manufacturer's protocol. BPEI (25 kDa) were prepared at the N/P ratio of 20/1. A549 cells were seeded onto six-well plates at a density of 5×105 cells per well in 2 mL of serum-free media and grown to reach 60-80% confluence. The culture medium was replaced with 2 mL of the transfection medium containing the Lipo2k or BPEI complex, followed by 6 h incubation at 37 °C. Then, the transfection medium was replaced with fresh complete RPMI 1640 (10% FBS), and the cells were allowed to grow for 48 h. After incubation, the cells were washed twice with DPBS (pH 7.4), and flow cytometry analysis was performed by the same method as described above.
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