Anti lif

The LLC and 293T cell lines were purchased from The American Type Culture Collection (ATCC, USA). Murine C2C12 myoblast cells were obtained from the Cell Bank/Stem Cell Bank, Chinese Academy of Sciences. Primers and oligonucleotides, including mmu-miR-29c-3p mimic, mmu-miR-29c-3p inhibitor, siRNA specific for LIF, and negative control were purchased from RiboBio, Guangzhou, China. Primary antibodies used for western blotting analysis include anti-Atrogin1 (Abcam, ab168372), anti-MuRF1 (proteintech, 55456-1-AP), anti-LC3 (proteintech, 14600-1-AP), anti-P62 (ABclonal, A19700), anti-GAPDH (Proteintech, 60004-1-Ig), anti-LIF (Abcam, ab113262), anti-p-p38 (Cell Signaling Technology, 4511T), anti-p38 (Cell Signaling Technology, 9212S), anti-p-STAT3 (ABclonal, AP0070), and anti-STAT3 (ABclonal, A1192). Primary antibodies used for Immunohistochemistry: anti-LIF (ABclonal, A1288). Primary antibodies used for fluorescence microscopy analysis: anti-MHC (R&D Systems, MAB4470).

The primary antibodies for anti-LIF, anti-KIM-1, and anti-NGAL were purchased from Abcam (Cambridge, MA, USA). Anti-C4b antibody was purchased from OriGene (Rockville, MD, USA). Anti-CFD and anti-CXCR6 antibody was purchased from MyBioSource (San Diego, CA, USA). Horseradish peroxidases (HRP)-conjugated secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Hematoxylin, eosin, and periodic acid-Schiff (PAS) stain kit were purchased from Dako (Glostrup, Denmark).