Zen lite blue edition software

Manufactured by Zeiss

ZEN lite blue edition is a software package for microscope image acquisition and basic image processing. It provides essential tools for capturing, visualizing, and analyzing microscope images.

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Lab products found in correlation

Fluoroshield mounting medium with dapi, by Abcam (1 mentions) Sc 8332, by Santa Cruz Biotechnology (1 mentions) Sc 11769 r, by Santa Cruz Biotechnology (1 mentions) Sc 8008, by Santa Cruz Biotechnology (1 mentions) Lsm 880, by Zeiss (1 mentions) Application suite las x, by Leica (1 mentions) Sp8 confocal lsm, by Leica (1 mentions) O dianisidine, by Merck Group (1 mentions) Stereo discovery v20 microscope, by Zeiss (1 mentions)

Close spelling variants of this product

ZEN software (2479 citations) Zen Blue software (487 citations) Zen Blue (223 citations) ZEN lite software (176 citations) ZEN lite (78 citations) ZEN blue edition software (59 citations) ZEN Blue Edition (56 citations) Zen Blue Lite (9 citations) Zen Blue lite software (8 citations) ZEN lite blue edition (6 citations)

3 protocols using zen lite blue edition software

Immunofluorescence Analysis of Hamster Jugal Mucosa

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Immunofluorescence was performed according to previously described methodology [22 (link)]. The hamster jugal mucosa samples (n = 5, per group) were deparafinized and washed with various concentrations of ethanol and PBS. Samples were incubated at 4°C overnight with rabbit polyclonal anti-MIF primary antibody (1:400; sc 20121, Santa Cruz Biotechnology), mouse monoclonal anti-NFkB primary antibody (1:200; sc-8008, Santa Cruz Biotechnology), rabbit polyclonal anti-SMAD primary antibody (1:400; sc-8332, Santa Cruz Biotechnology) and rabbit polyclonal anti-pSMAD primary antibody (1:400; sc-11769-R, Santa Cruz Biotechnology). Then, the slides were washed in PBS/0.2% triton X-100 for 5 min, and incubated with Alexa Fluor 488 (1:500 in 1% BSA) and and were mounted with Fluoroshield Mounting Medium with DAPI (abcam®, UK). The quantitative analysis of the fluorescence intensity was determined from digital images of at least 3 different areas of each section (five animals per group), on 100x magnification, using the Zeiss ZEN lite blue edition software.

Ribeiro S.B., de Araújo A.A., de Araújo Júnior R.F., Brito G.A., Leitão R.C., Barbosa M.M., Garcia V.B., Medeiros A.C, & de Medeiros C.A. (2017). Protective effect of dexamethasone on 5-FU-induced oral mucositis in hamsters. PLoS ONE, 12(10), e0186511.

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Imaging Cellular Localization with Fluorescent Markers

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Cells were fixed with 4% formaldehyde and washed 3 times with 1× phosphate-buffered saline. Fixed cells were then stained with DAPI and mounted on a microscope slide for imaging. Nuc1-GFP images were taken using the Zeiss LSM 880 with Airyscan confocal laser scanning microscope (LSM) on the Fast module and processed with ZEN lite (blue edition) software from Carl Zeiss Microscopy. Por1-GFP/Por2-mCherry images were acquired with the Leica Sp8 confocal LSM and processed with the Leica Application Suite (LAS X) software package. Deconvolution was applied to all images obtained by microscopy.

Gao J., Chau S., Chowdhury F., Zhou T., Hossain S., McQuibban G.A, & Meneghini M.D. (2019). Meiotic viral attenuation through an ancestral apoptotic pathway. Proceedings of the National Academy of Sciences of the United States of America, 116(33), 16454-16462.

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Frog Embryo Cranial Hemorrhage Assessment

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To assess cranial hemorrhage, frog embryos were collected at stage 39 (dpf 3) and anesthetized with benzocaine. Cranial hemorrhage was scored by visual inspection using a Zeiss SteREO Discovery V20 microscope. Whole-embryo o-Dianisidine (Sigma-Aldrich, D9143) staining was performed to confirm cranial hemorrhage phenotype at stage 39 and vascular dysmorphology at stage 46 (dpf 4). In brief, embryos were collected at corresponding stages and stained for 15 min in the dark in a solution consisting of 0.6 mg ml⁻¹ of o-Dianisidine, 0.01 M sodium acetate (pH 4.5), 0.65% H₂O₂ and 40% (v/v) ethanol. Embryos were examined by a Zeiss SteREO Discovery V20 microscope. Images were opened with Fiji (ImageJ) Color Mode: ‘Default’ and ‘Autoscale’ using either CZI files or TIFF files generated by Zeiss ZEN lite blue edition software. Contrast enhancement was performed using the ‘Enhance Contrast’ application in the ‘Process’ tab by performing 0.3% pixel saturation in whole image if necessary. Both the ‘autoscale’ and ‘enhanced contrast’ options were applied to all channels and the whole image.

Barak T., Ristori E., Ercan-Sencicek A.G., Miyagishima D.F., Nelson-Williams C., Dong W., Jin S.C., Prendergast A., Armero W., Henegariu O., Erson-Omay E.Z., Harmanci A.S., Guy M., Gültekin B., Kilic D., Rai D.K., Goc N., Aguilera S.M., Gülez B., Altinok S., Ozcan K., Yarman Y., Coskun S., Sempou E., Deniz E., Hintzen J., Cox A., Fomchenko E., Jung S.W., Ozturk A.K., Louvi A., Bilgüvar K., Connolly ES J.r., Khokha M.K., Kahle K.T., Yasuno K., Lifton R.P., Mishra-Gorur K., Nicoli S, & Günel M. (2021). PPIL4 is essential for brain angiogenesis and implicated in intracranial aneurysms in humans. Nature medicine, 27(12), 2165-2175.

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