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Tazmetostat

Manufactured by Selleck Chemicals
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Sourced in United States

Tazmetostat is a laboratory equipment product used for scientific research. It is a chemical compound that functions as an epigenetic modifier. The core function of Tazmetostat is to inhibit the enzymatic activity of EZH2, a histone methyltransferase involved in gene expression regulation.

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Lab products found in correlation

Mak 683, by Avantor (2 mentions) Cpi 1205, by Selleck Chemicals (2 mentions) I bet151, by Selleck Chemicals (1 mentions) Abbv744, by Selleck Chemicals (1 mentions) Otx015, by Selleck Chemicals (1 mentions) Ml324, by Selleck Chemicals (1 mentions) Anbl 6, by American Type Culture Collection (1 mentions) Kms11, by American Type Culture Collection (1 mentions) Carfilzomib, by Selleck Chemicals (1 mentions) Panobinostat, by Selleck Chemicals (1 mentions) Vorinostat, by Selleck Chemicals (1 mentions) Ricolinostat, by Selleck Chemicals (1 mentions) Romidepsin, by Selleck Chemicals (1 mentions) Entinostat, by Selleck Chemicals (1 mentions) Gsk lsd1, by Selleck Chemicals (1 mentions) Bortezomib, by Selleck Chemicals (1 mentions) Alamar blue, by Thermo Fisher Scientific (1 mentions) Mab374, by Thermo Fisher Scientific (1 mentions) Polyvinyl difluoride membrane, by Merck Group (1 mentions)

3 protocols using tazmetostat

1

Multiple Myeloma Cell Lines and Compounds

Cited 2 times
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Cell lines (KMS11, OPM2, and ANBL6) were purchased from American Type Culture Collection (Manassas, VA, USA). MM.1S BzR cells were a gift from Dr. Brian Van Ness (U. of Minnesota) and have been described previously [25 (link)]. LTI6426 was provided by Leukogene Therapeutics, Inc. (Charleston, SC) with purity and identity that was confirmed by liquid chromatograph–mass spectrometry (LC–MS) and nuclear magnetic resonance (NMR) as shown previously [16 (link)]. Bortezomib (Catalog No. S1013), carfilzomib (Catalog No. S2853), panobinostat (Catalog No. S1030), vorinostat (Catalog No. S1047), ricolinostat (Catalog No. S8001), entinostat (Catalog No. S1053), romidepsin (Catalog No. S3020), tazmetostat (Catalog No. S7128), ML324 (Catalog No. S7296), JQ1 (Catalog No. S7110), OTX015 (Catalog No. S7360), ABBV744 (Catalog No. S8723), IBET151 (Catalog No. S2780), and GSK-LSD1 (Catalog No. S7574) were purchased from Selleck Chemicals (Houston, TX, USA).
Robinson R.M., Basar A.P., Reyes L., Duncan R.M., Li H, & Dolloff N.G. (2022). PDI inhibitor LTI6426 enhances panobinostat efficacy in preclinical models of multiple myeloma. Cancer Chemotherapy and Pharmacology, 89(5), 643-653.
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2

Cytotoxicity Assay of TNBC Inhibitors

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TNBC cell lines were plated in quadruplicate at 2,000 cells/well into 96-well plates. Serial dilutions of tazmetostat (Selleck Chemicals, #S7128), CPI-1205 (Selleck Chemicals, #S8353), or MAK-683 (VWR, #B1972) were added to wells after 24 h. Cell viability was determined after 4 days of treatment by measuring the fluorescence of metabolic reduction of AlamarBlue (ThermoFisher, #DAL1100), per the manufacturer’s protocol. Average viability was determined relative to vehicle control from three independent experiments.
Lehmann B.D., Colaprico A., Silva T.C., Chen J., An H., Ban Y., Huang H., Wang L., James J.L., Balko J.M., Gonzalez-Ericsson P.I., Sanders M.E., Zhang B., Pietenpol J.A, & Chen X.S. (2021). Multi-omics analysis identifies therapeutic vulnerabilities in triple-negative breast cancer subtypes. Nature Communications, 12, 6276.
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3

Immunoblotting of Epigenetic Markers in TNBC

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TNBC cell lines were lysed in 100 mm plates 0, 1, 2, 3, or 5 days after treatment with a single 10 μM treatment of tazmetostat (Selleck Chemicals, #S7128), CPI-1205 (Selleck Chemicals, #S8353) or MAK-683 (VWR, #B1972). All cells were lysed in a RIPA buffer supplemented with protease and phosphatase inhibitors. Cell lysates (40 μg) were separated on polyacrylamide gels and transferred to polyvinyl difluoride membranes (Millipore). Immunoblotting was performed using anti-H3K27me3 (1:1000, Cell Signaling, 9733S), anti-histone 3 (1:5000, Abcam, ab1791), anti-HLA-A/B/C (1:1000, Santa Cruz, sc-52810), anti-GAPDH (1:5000, EMD Millapore, MAB374), and anti-vinculin (1:2000, ThermoFisher, 700062).
Lehmann B.D., Colaprico A., Silva T.C., Chen J., An H., Ban Y., Huang H., Wang L., James J.L., Balko J.M., Gonzalez-Ericsson P.I., Sanders M.E., Zhang B., Pietenpol J.A, & Chen X.S. (2021). Multi-omics analysis identifies therapeutic vulnerabilities in triple-negative breast cancer subtypes. Nature Communications, 12, 6276.
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