Human transferrin apoform transferrin

Manufactured by Fujifilm

Human transferrin apoform (transferrin) is a protein found in the blood that binds and transports iron. It is a key component in various laboratory procedures and research applications.

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Lab products found in correlation

Arthrobacter ureafaciens neuraminidase, by Nacalai Tesque (2 mentions) Streptococcus pneumoniae β galactosidase, by New England Biolabs (2 mentions) Human haptoglobin, by Merck Group (1 mentions) Human α1 antitrypsin, by Merck Group (1 mentions) Human α1agp, by Merck Group (1 mentions) Human fibrinogen, by Fujifilm (1 mentions)

2 protocols using human transferrin apoform transferrin

Glycoprotein Deglycosylation Protocol

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Human transferrin apoform (transferrin) (Wako), human α1AGP (Sigma; catalog no.: G9885), human fibrinogen (Fujifilm; catalog no.: 061-03691), human haptoglobin (Sigma; catalog no.: H3536), human α1-antitrypsin (Sigma; catalog no.: A9024), and human IgG (Wako; catalog no.: 143-09501) were purchased and used for the assays. One milligram of α1AGP, transferrin, fibrinogen, haptoglobin, α1-antitrypsin, or IgG was digested with 3.8 munit/μl of Arthrobacter ureafaciens neuraminidase (Nacalai Tesque) and 1.2 unit/μl of Streptococcus pneumoniae β-galactosidase (New England Biolabs) in 87 mM sodium acetate buffer (pH 4.5) at 37 °C overnight.

Osada N., Nagae M., Nakano M., Hirata T, & Kizuka Y. (2022). Examination of differential glycoprotein preferences of N-acetylglucosaminyltransferase-IV isozymes a and b. The Journal of Biological Chemistry, 298(9), 102400.

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Glycosidase Treatment and Heat Denaturation of Glycoproteins

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Human α1AGP (G9885, Sigma), human transferrin apoform (transferrin) (Wako), and chicken egg SGP (α2,6-SGP) (Fushimi) were purchased and used for the assays. A total of 500 μg of α1AGP, transferrin, or SGP was digested with 0.25 unit/ml of Arthrobacter ureafaciens neuraminidase (Nacalai Tesque) and 80 unit/ml of Streptococcus pneumoniae β-galactosidase (New England Biolabs) in 87 mM sodium acetate buffer (pH 4.5) at 37 °C overnight. For heat denaturation, glycosidase-treated α1AGP was incubated at 60, 80, or 100 °C for 10 min.

Osuka R.F., Hirata T., Nagae M., Nakano M., Shibata H., Okamoto R, & Kizuka Y. (2022). N-acetylglucosaminyltransferase-V requires a specific noncatalytic luminal domain for its activity toward glycoprotein substrates. The Journal of Biological Chemistry, 298(3), 101666.

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