Dextran sodium sulfate (DSS) was purchased from MP Biomedicals (Irvine, CA, United States), and salazosulfapyridine (SASP) was purchased from Xinyi Pharmaceutical Group (Shanghai, China). BT2 was obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). The LEGENDplex™ Multi-Analyte Flow Assay Kit was supplied by BioLegend (San Diego, CA, United States). The BCA Protein Quantification Kit and Supper ECL Detection Reagent were purchased from Yeasen Biotechnology Co., Ltd. (Shanghai, China). The antibodies used for these experiments were as follows: Ribosomal protein S6 (S6) [1:1000, #2317; Cell Signaling Technology (CST), Danvers, MA, United States], phosphorylated S6Ser235/236 (p-S6, 1:1000, 4858; CST), eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) (9644, 1:1000; CST), p-4EBP1Thr37/46 (1:1000, 2855; CST), COX-2 (1:1000, 12282; CST), mTOR (1:1000, 66878-1-Ig; Proteintech, Rosemont, IL, United States), p-mTORSer2448 (1:1000, 67778-1-Ig; Proteintech), branched-chain amino transferase 2 (BCAT2) (1:1000, 16417-1-AP; Proteintech), rabbit antibody (1:10000, SA00001-2; Proteintech), mouse antibody (1:10000, SA00001-1; Proteintech), BCKDK (1:500, 374424; Santa Cruz, Dallas, TX, United States), and branched-chain α–keto acid dehydrogenase (BCKDHA) (1:500, 271538; Santa Cruz).
Bckdha
Manufactured by Santa Cruz Biotechnology
Sourced in United States
BCKDHA is an enzyme that plays a key role in the breakdown of certain amino acids. It is a component of the branched-chain alpha-keto acid dehydrogenase complex, which is responsible for the oxidative decarboxylation of the branched-chain alpha-keto acids derived from leucine, isoleucine, and valine.
Automatically generated - may contain errors
Lab products found in correlation
Legendplex multi analyte flow assay kit, by BioLegend (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Sa00001 1, by Proteintech (1 mentions)
Sa00001 2, by Proteintech (1 mentions)
Bca protein quantification kit, by Yeasen (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
P bckdha, by Abcam (1 mentions)
2 protocols using bckdha
1
Molecular Mechanisms of DSS-Induced Colitis
2
Protein Expression Analysis by Western Blot
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Western blotting (immunoblotting and detection) was performed as previously described. 29 Protein isolates were extracted from tissue using RIPA buffer containing protease and phosphatase inhibitor cocktail (Thermo ), BCKDHA (Santa Cruz, sc-67200), p-BCKDHA (Ser293) (Abcam, ab200577), CPT1 (Alpha Diagnostics, CPT1M11-A), HACL1 (Sigma, HPA035496), p-ACC (Ser79) (CST, 3661), ACC (CST, 3662), FASN (CST, 3189), and were normalized to either ATUB (Sigma, T6074) or GAPDH (Ambion, AM4300). Western blot densitometry was quantified using ImageJ software and data were normalized to control group and set to a value of 1.
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