Log In Sign up
The largest database of trusted experimental protocols

Violetfluor 450 α ly 6g gr1 rb6 8b5

Manufactured by Cytek Biosciences
Visit Supplier

Violetfluor 450-α-Ly-6G (GR1) (RB6-8B5) is a fluorescent-labeled antibody that binds to the Ly-6G (GR1) antigen. It is designed for use in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd11b microbead kit, by Miltenyi Biotec (2 mentions) Facsaria 2 sorp, by BD (2 mentions) Fc block α cd16 32, by BioLegend (2 mentions) Pe cy7 α cd19 1d3, by BD (2 mentions) Fitc α cd11b m1 70, by BD (2 mentions) Bv510 α f4 80 bm8, by BioLegend (2 mentions) Bv711 α siglec f e50 2440, by BD (2 mentions) Alexafluor 647 α cd102 3c4 mic2 4, by BioLegend (2 mentions) Ls column, by Miltenyi Biotec (2 mentions)

2 protocols using violetfluor 450 α ly 6g gr1 rb6 8b5

1

Isolation and Characterization of Murine Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three to four mice were pooled for each sample. PECs were collected and washed. Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend) and then PE-Cy7-α-CD19 (1D3, Tonbo), Fitc-α-CD11b (M1/70, BD Biosciences), BV510-α-F4/80 (BM8, Biolegend), BV711-α-Siglec F (E50-2440, BD Biosciences), Violetfluor 450-α-Ly-6G (GR1) (RB6-8B5, Tonbo), and Alexafluor 647-α-CD102 (3C4 (MIC2/4), Biolegend). Samples were run on a BD FACS Aria II SORP and collected in D10 media (for LD-PCR). Afterward, the samples were counted and frozen for LD-PCR (in media).
Zarek C.M., Dende C., Coronado J., Pendse M., Dryden P., Hooper L.V, & Reese T.A. (2023). Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages. PLOS Pathogens, 19(10), e1011691.
+ Open protocol
+ Expand
2

Isolation and Characterization of Murine Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three to four mice were pooled for each sample. PECs were collected and washed. Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend) and then PE-Cy7-α-CD19 (1D3, Tonbo), Fitc-α-CD11b (M1/70, BD Biosciences), BV510-α-F4/80 (BM8, Biolegend), BV711-α-Siglec F (E50-2440, BD Biosciences), Violetfluor 450-α-Ly-6G (GR1) (RB6-8B5, Tonbo), and Alexafluor 647-α-CD102 (3C4 (MIC2/4), Biolegend). Samples were run on a BD FACS Aria II SORP and collected in D10 media (for LD-PCR). Afterward, the samples were counted and frozen for LD-PCR (in media).
Zarek C.M., Dende C., Coronado J., Pendse M., Dryden P., Hooper L.V, & Reese T.A. (2023). Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages. PLOS Pathogens, 19(10), e1011691.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!