In the fall of 2018, saliva and oropharyngeal swabs were collected from college students of Hogeschool Utrecht (n = 300). After signing informed consent, students self-collected saliva by spitting 1 ml into a 15 ml tube (Greiner, Kremsmünster, Austria). Next, a study nurse swabbed student’s posterior pharyngeal wall with a nylon swab (FLOQSwabs, COPAN, Brescia, Italy) to collect an oropharyngeal sample. Immediately after collection, saliva (approximately 50 µl) and oropharyngeal swab were used to inoculate Neisseria Selective Medium PLUS agar plates (NS-agar, Oxoid, Badhoevedorp, the Netherlands) and within 20 min plates were placed in a 37 °C, 5% CO2 incubator. Once all samples have been collected, cultured plates were transported at room temperature to the laboratory.
Neisseria selective medium plus agar plates ns agar
Manufactured by Thermo Fisher Scientific
Sourced in Netherlands
Neisseria Selective Medium PLUS agar plates (NS-agar) are a culture medium used for the isolation and identification of Neisseria species, particularly Neisseria gonorrhoeae and Neisseria meningitidis, from clinical specimens. The medium contains selective agents that inhibit the growth of other bacteria, allowing for the selective isolation of Neisseria species.
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Lab products found in correlation
2 protocols using neisseria selective medium plus agar plates ns agar
1
Oropharyngeal Sampling and Culturing Protocol
2
Oropharyngeal Sampling of College Students
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In the fall of 2018, saliva and oropharyngeal swabs were collected from college students of Hogeschool Utrecht (n=300). After signing informed consent, students self-collected saliva by spitting 1ml into a 15ml tube (Greiner, Kremsmünster, Austria). Next, a study nurse swabbed student's posterior pharyngeal wall with a nylon swab (FLOQSwabs, COPAN, Brescia, Italy) to collect an oropharyngeal sample. Immediately after collection, saliva (approximately 50 µl) and oropharyngeal swab were used to inoculate Neisseria Selective Medium PLUS agar plates (NS-agar; Oxoid, Badhoevedorp, the Netherlands) and within 20 minutes plates were placed in a 37°C, 5% CO2 incubator. Once all samples have been collected, cultured plates were transported at room temperature to the laboratory.
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