Adult NC cells were cultured on poly-L ornithine (100 ng/ml; Sigma)/laminin (10 mg/ml; EMD Millipore, Billerica, MA) coated dishes and exposed to neuron differentiation media containing Neurobasal plus medium (Thermo Fisher Scientific) with BMP2 (10 ng/ml; R&D systems, Minneapolis, MN), SB431542 (10 μM), B27 plus (Thermo Fisher Scientific), N2 supplement (R&D systems), Brain-derived neurotrophic factor (BDNF; 10 ng/ml; Thermo Fisher Scientific), Glial cell-derived neurotrophic factor (GDNF;10 ng/ml; Sigma), Nerve growth factor (NGF; 10 ng/ml; R & D systems), Neurotrophin 3 (NT3; 10 ng/ml; Sigma), ascorbic acid (200 μM; Sigma) and cyclic adenosine monophosphate (0.5 mM cAMP; Sigma), CHIR 99021 (0.5 μM, only on day 1; Sigma), 2% FBS (from day 1–5), IWP-4 (100 nM days 4–6; 1 μM thereafter; Tocris Bioscience, Minneapolis, MN).
Neurotrophin 3 nt3
Manufactured by Merck Group
Neurotrophin 3 (NT3) is a protein that plays a critical role in the development and maintenance of the nervous system. It is a member of the neurotrophin family, which also includes nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-4 (NT-4). NT3 is involved in the survival, differentiation, and function of various types of neurons, including sensory and motor neurons. It is commonly used in research applications focused on understanding and addressing neurological conditions.
Automatically generated - may contain errors
Lab products found in correlation
Poly l ornithine, by Merck Group (2 mentions)
Cyclic adenosine monophosphate, by Merck Group (1 mentions)
N2 supplement, by R&D Systems (1 mentions)
Nerve growth factor (ngf), by R&D Systems (1 mentions)
Brain derived neurotrophic factor bdnf, by Thermo Fisher Scientific (1 mentions)
Chir99021, by Merck Group (1 mentions)
Glial cell derived neurotrophic factor gdnf, by Merck Group (1 mentions)
Sb431542, by Thermo Fisher Scientific (1 mentions)
B 27 plus, by Thermo Fisher Scientific (1 mentions)
Neurobasal plus medium, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Bmp 2, by R&D Systems (1 mentions)
Laminin, by Merck Group (1 mentions)
Brain derived neurotrophic factor (bdnf), by Merck Group (1 mentions)
β tubulin 3 tuj1, by BioLegend (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
2 protocols using neurotrophin 3 nt3
1
Differentiation of Adult Neural Cells
2
Differentiation of SHED into Spiral Ganglion Neurons
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SHED were seeded onto poly-L-ornithine (Sigma) and laminin (Sigma) coated 24-well plates. The cells were cultured for 14 days in spiral ganglion neurons induction medium consisting of DMEM/F12, 20 ng/ml neurotrophins brain-derived neurotrophic factor (BDNF), 20 ng/ml neurotrophin-3 (NT-3), 20 ng/ml glial cell-derived neurotrophic factor (GDNF) (all from Sigma), supplemented with 2% N-2 (Gibco), 2% B-27 supplement, 100 U/ml Penicillin, and 100 μm/ml Streptomycin. The medium was changed every other day. The differentiated cells were stained with β-tubulin III (Tuj1; BioLegend, San Diego, CA, USA) and Tropomyosin receptor kinase B (TrkB; Santa Cruz Biotechnology, Inc, Dallas, TX, USA). Nuclei were counterstained with 20 μg/ml 4′,6-diamidino-2-phenylindole (DAPI; Sigma).
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