Non-parenchymal liver cells (NPCs) were isolated as previously described with slight modifications [32] (link). Cells (0.3–0.5 × 106 cells/test) were incubated with CD45-FITC (rat IgG, Beckman Coulter, Madrid, Spain), CD11b-(Mac1)-PECy7 (rat IgG2Bk anti-mouse, eBioscience, ThermoFisher Scientific, Waltham, MA, USA), F4/80-APC (rat IgG2ak, eBioscience), Ly6G-PE (rat IgG2ak, Pharmingen, San José, CA, USA), CD3-PECy7 (Hamster IgG, eBioscience), NK1.1-APC (mice IgG2ak anti-mouse, Pharmingen), CD8a-PE (2.4G2, Cultek, Madrid Spain), F4/80-PE (rat IgG2ak, eBioscience), Ly6C-FITC (rat IgMk, anti-mouse, Pharmingen), and CCR2-APC (rat IgG2B, R&D Systems, Minneapolis, MN, USA) or their corresponding isotype controls for 20 min at room temperature. Flow cytometry data were acquired with a FACSCanto II (BD Biosciences, Madrid, Spain) and data analysis was performed using Cytomics FC500 with the CXP program.
Cd11b mac1 pecy7
Manufactured by Thermo Fisher Scientific
Sourced in Spain, United States
CD11b-(Mac1)-PECy7 is a monoclonal antibody conjugated with Phycoerythrin-Cyanine 7 (PECy7) dye. It is used to detect the CD11b (Mac-1) antigen, which is expressed on the surface of myeloid cells, such as monocytes, macrophages, and granulocytes.
Automatically generated - may contain errors
Lab products found in correlation
Cd45 fitc, by Beckman Coulter (2 mentions)
Rat igg2b, by R&D Systems (2 mentions)
F4 80 pe, by Thermo Fisher Scientific (2 mentions)
Cd3 pe cy7, by Thermo Fisher Scientific (1 mentions)
F4 80 apc, by Thermo Fisher Scientific (1 mentions)
Nk1.1 apc, by BD (1 mentions)
Hamster igg, by Thermo Fisher Scientific (1 mentions)
Ly6c fitc, by BD (1 mentions)
Ly6g pe, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Ly6c fitc, by Thermo Fisher Scientific (1 mentions)
Rat igg2ak, by Thermo Fisher Scientific (1 mentions)
Perfect count microspheres, by Cytognos (1 mentions)
2 protocols using cd11b mac1 pecy7
1
Liver Non-Parenchymal Cell Isolation
2
Quantification of Immune Cell Populations
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NPC cells (0.3-0.5 × 10 6 cells/test) were incubated for 20 min at RT in the dark with the following antibodies (5 μg/ml): CD11b-Mac1-PECy7 (rat IgG2bk, anti-mouse eBioscience, San Diego CA, USA), CD45-FITC (rat IgG, Beckman), F4/80-PE (ratIgG2ak, eBioscience), Ly6C-FITC (rat IgMk, anti-mouse, Pharmingen, San José, CA, USA) and CCR2-APC (ratIgG2B, R&DSystems, Minneapolis, MN, USA) or their corresponding isotype controls. After three washes, Perfect-Count microspheres (Cytognos, Salamanca, Spain) were added to quantify the exact number of cells. Flow cytometry analysis was performed using Cytomics FC500 with the CXP program. In Supplementary Fig. 1 is shown the efficiency of isolation of immune cell population in RCD and MCD groups.
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