Apoptosis assay was performed using a fluorescein isothiocyanate (FITC)-conjugated annexin V kit (BD Pharmingen, San Diego, CA, USA) according to the manufacturer’s instructions. Fluorescence was counted using a NucleoCounter NC-3000 image cytometer (ChemoMetec, Allerød, Denmark) [31 (link)].
Fitc conjugated annexin 5 kit
Manufactured by BD
Sourced in United States
The (FITC)-conjugated annexin V kit is a laboratory reagent used to detect and quantify apoptotic cells. Annexin V is a protein that binds to phosphatidylserine, which is externalized on the surface of cells undergoing apoptosis. The FITC (fluorescein isothiocyanate) label allows the detection of annexin V binding using fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using fitc conjugated annexin 5 kit
1
Apoptosis Evaluation via Annexin V Assay
2
Annexin V and Propidium Iodide Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was analyzed by detecting phosphatidylserine (PS) on the outer leaflet of the plasma membrane using a FITC-conjugated annexin V kit (BD Pharmingen; San Diego, CA, USA), according to the manufacturer’s instructions. Dead cells were counterstained using propidium iodide (PI). Annexin V- and PI-stained cells were counted via flow cytometry using a NucleoCounter NC-3000, as described previously (20 (link)).
3
MTT Assay for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), RPMI-1640, phosphate buffer saline (PBS), bovine serum albumin (BSA) and ethylenediaminetetraacetic acid (EDTA) were all purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Foetal bovine serum (FBS) was purchased from PAA (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) and was heat-activated for 30 min in a 56 °C water bath prior to use. Tryple E from Gibco Invitrogen (Gibco, Invitrogen, Thermo Scientific, Hudson, NH, USA), DMSO (Thermo Scientific, Hudson, NH, USA). 96-well and 6-well plates, FITC-conjugated Annexin V kit were purchased from (BD Biosciences, San Jose, CA, USA).
4
Neutrophil Adhesion and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neutrophils were analysed by flow cytometry to quantify the percentage of neutrophils: a) remaining morphologically intact, b) showing markers of apoptosis, c) containing myeloperoxidase, after 18 h of adhesion to fibrinogen coated surfaces. To this end, neutrophils were detached from fibrinogen by brief exposure to EDTA/EGTA (both 5 mMol/L). Neutrophil apoptosis was quantified by a FITC-conjugated Annexin-V Kit according to the manufacturer's instruction (BD-Biosciences, USA). Intracellular myeloperoxidase content was measured by the "MPO-FITC" Kit according to manufacturer instruction (Beckman Coulter, Milan, Italy).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!