T84 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). DMEM/Ham’s F-12 media, Minimum Essential Medium Eagle, Spinner Modification (S-MEM), penicillin, and streptomycin were purchased from Gibco, ThermoFisher Scientific (Waltham, MA, USA). MTT was purchased from Bio Basic Inc. (Markham, Canada). DMSO, FITC-dextran (molecular weight of 4400 Da,) and forskolin were purchased from Sigma Aldrich (St. Louis, MO, USA). Afatinib, CFTR-172, and Compound C were purchased from Merck Millipore (Darmstadt, Germany). Antibodies including AMPK-α, p-AMPK-α, ACC, p-ACC, AKT, p-AKT, ERK42/44, p-ERK42/44, and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA).
Erk42 44
Manufactured by Cell Signaling Technology
Sourced in United States
ERK42/44 is a laboratory equipment product that detects the levels of extracellular signal-regulated kinases (ERK) 42 and 44. ERK42/44 is used to measure the activation of these important cellular signaling proteins.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Dmem ham s f12 media, by Thermo Fisher Scientific (2 mentions)
P erk42 44, by Cell Signaling Technology (2 mentions)
P akt, by Cell Signaling Technology (2 mentions)
Fitc dextran, by Merck Group (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Bio Basic (2 mentions)
T84 cells, by American Type Culture Collection (2 mentions)
Forskolin, by Merck Group (1 mentions)
P ampkα, by Cell Signaling Technology (1 mentions)
P acc, by Cell Signaling Technology (1 mentions)
Minimum essential medium eagle, by Thermo Fisher Scientific (1 mentions)
Ampkα, by Cell Signaling Technology (1 mentions)
Compound c, by Merck Group (1 mentions)
Wortmannin, by MedChemExpress (1 mentions)
2 protocols using erk42 44
1
T84 Cell Proliferation and Signaling
2
T84 Cell Culture and Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
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T84 cells obtained from American Type culture Collection (Manassas, VA, USA). DMEM/Ham’s F-12 media, penicillin, streptomycin were purchased from Gibco, Thermofisher Scientific, Waltham, MA, USA). Wortmannin and SC79 were purchased from MedChemExpress (New jersey, USA). MTT was purchased from Bio Basic Inc. (Markham, Canada). DMSO and FITC-dextran (MW 4000 Da) were purchased from Sigma Aldrich (St. Louis, MO, USA). Antibodies such as AKT, p-AKT, ERK42/44, p-ERK42/44 and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals used were of at least reagent grade and were purchased commercially.
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