Riboflavin 5 monophosphate sodium salt fmn

Chloramphenicol (≥98%; C0378-5G), thiamphenicol (>99.9%; T0261-15), and kanamycin sulfate (KAN) (K1377) were purchased from Sigma-Aldrich and prepared as 50 mg/mL stock solutions in ethanol (amphenicols) or water, filter sterilized, and stored at −20°C. Bratton-Marshall reagent (N-[1-naphthyl]ethylenediamine HCl) (98+%; AC423990250), sodium nitrite (97% minimum; AA1424422), ammonium sulfamate (98+%; AC423390050), riboflavin 5′-monophosphate sodium salt (FMN) (93.0+%; R00235G), and florfenicol (NC1617017) were purchased from Fisher Scientific and prepared as described below. β-Nicotinamide reduced tetrasodium salt (NADPH) was purchased from Dot Scientific (>93%; DSN20140-0.1), stored dry at −20°C, and prepared fresh for all experiments. Amino-Chloramphenicol was purchased from Toronto Research Chemicals (A622670) as an HCl salt and prepared by dissolution in water. Amino-Chloramphenicol solutions were stored at −20°C for less than 1 month. Sch-24893 (Fig. 1b) was synthesized by the Northwestern University ChemCore, validated by proton and carbon NMR in dimethyl sulfoxide (DMSO) (Fig. S1A and B), and prepared as a 50 mg/mL stock solution in ethanol.

Chloramphenicol (≥98%; C0378-5G), thiamphenicol (>99.9%; T0261-15), and kanamycin sulfate (KAN) (K1377) were purchased from Sigma-Aldrich and prepared as 50 mg/mL stock solutions in ethanol (amphenicols) or water, filter sterilized, and stored at −20°C. Bratton-Marshall reagent (N-[1-naphthyl]ethylenediamine HCl) (98+%; AC423990250), sodium nitrite (97% minimum; AA1424422), ammonium sulfamate (98+%; AC423390050), riboflavin 5′-monophosphate sodium salt (FMN) (93.0+%; R00235G), and florfenicol (NC1617017) were purchased from Fisher Scientific and prepared as described below. β-Nicotinamide reduced tetrasodium salt (NADPH) was purchased from Dot Scientific (>93%; DSN20140-0.1), stored dry at −20°C, and prepared fresh for all experiments. Amino-Chloramphenicol was purchased from Toronto Research Chemicals (A622670) as an HCl salt and prepared by dissolution in water. Amino-Chloramphenicol solutions were stored at −20°C for less than 1 month. Sch-24893 (Fig. 1b) was synthesized by the Northwestern University ChemCore, validated by proton and carbon NMR in dimethyl sulfoxide (DMSO) (Fig. S1A and B), and prepared as a 50 mg/mL stock solution in ethanol.