Log In Sign up
The largest database of trusted experimental protocols

Atp5a1

Manufactured by Merck Group
Visit Supplier
Sourced in United States

ATP5A1 is a protein subunit of the ATP synthase complex, which is responsible for the production of ATP in the mitochondria of eukaryotic cells. It plays a key role in the process of oxidative phosphorylation, which is the final stage of cellular respiration. ATP5A1 is essential for the proper functioning of the ATP synthase complex and the generation of cellular energy.

Automatically generated - may contain errors

Lab products found in correlation

Anti pkm2, by Cell Signaling Technology (1 mentions) Rna binding protein immunoprecipitation kit, by Merck Group (1 mentions) Gapdh, by Merck Group (1 mentions) β tubulin, by Merck Group (1 mentions) Hpa001520, by Merck Group (1 mentions)

2 protocols using atp5a1

1

RNA Immunoprecipitation of PKM2 and ATP5A1

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA immunoprecipitation assays were performed using RNA-Binding Protein Immunoprecipitation kit (Millipore, Burlington, MA) according to the manufacturer's instructions. 5 μg Rabbit anti-PKM2 (Cell Signaling Technology, Danvers, MA) and ATP5A1 (Sigma, St. Louis, MO) antibodies were used to perform RNA immunoprecipitation, respectively. The enrichment of LINC01554 was determined by RT-PCR.
Zheng Y.L., Li L., Jia Y.X., Zhang B.Z., Li J.C., Zhu Y.H., Li M.Q., He J.Z., Zeng T.T., Ban X.J., Yuan Y.F., Li Y, & Guan X.Y. (2019). LINC01554-Mediated Glucose Metabolism Reprogramming Suppresses Tumorigenicity in Hepatocellular Carcinoma via Downregulating PKM2 Expression and Inhibiting Akt/mTOR Signaling Pathway. Theranostics, 9(3), 796-810.
+ Open protocol
+ Expand
2

Primary Antibody Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were purchased from the manufacturers: ATP5A1 (Sigma-Aldrich, SAB4502040), ATP5B (Sigma, HPA001520), GAPDH (Sigma-Aldrich, G8795), b-tubulin (Sigma-Aldrich, T5201), HaloTag® (Promega, Madison, WI, USA, G921A), and V5 (Nacalai Tesque, V5005).
Ueda K, & Suwanmanee Y. (2022). ATP5B Is an Essential Factor for Hepatitis B Virus Entry. International Journal of Molecular Sciences, 23(17), 9570.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!