Immunohistochemical analysis of tumor tissue sections from the ERC cohort was carried out according to the standard technique with a peroxidase detection system with DAB on an automatic Leica BOND III IHC staining system using Leica reagents and protocols. Antibodies to CD45 (PA0042, Leica), CD3 (PA0553, Leica), CD4 (PA0427, Leica), CD8 (PA0183, Leica), and CD68 (PA0273, Leica) were used. All histological slides were scanned using a Leica Aperio AT2 system at 20× magnification for further analysis.
Pa0042
Manufactured by Leica
Sourced in Germany
The PA0042 is a high-precision laboratory instrument manufactured by Leica. It is designed for accurate measurement and analysis applications in scientific research and industrial settings. The device's core function is to provide reliable and consistent performance for the intended use, without further interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pa0042
1
Immunohistochemical Profiling of Tumor Tissue
2
Double Sequential IHC Staining of CD248, ERG, and CD45
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We performed double sequential IHC staining on the Leica Biosystems Bond-III automated staining system, using either the rabbit anti-Hu CD248 (clone E9Z7O, Ref 47948S Cell Signaling technology, Danvers, MA, USA), the rabbit anti-Hu ERG (clone EPR3864, Ventana, Cupertino, CA, USA), or the mouse antiCD45 (mouse monoclonal PA0042, Leica, Wetzlar, Germany). We used a paraffin wax RA tissue section and antigen retrieval was achieved using an EDTA-based solution (ER2, Leica). Revelation was carried out using BOND Polymer Refine Detection kits (Ref Ds9390 and Ds9800) according to the manufacturer’s instructions (Leica, Paris, France).
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