Pathogen-free, adult male and female Sprague-Dawley rats (100-250 g, Envigo, Placentia, CA) were kept in light (12-h light: 12-h dark cycle; lights on at 07:00 h) and temperature (23 ± 3°C) controlled rooms, with standard rodent chow and water ad libitum. Pathogen-free, male Sprague Dawley rat pups (postnatal 10-15 days; Envigo, Placentia, CA) were used for electrophysiological recordings in spinal cord slices. All animal use was conducted in accordance with the National Institutes of Health guidelines, and the study was conducted in strict accordance with recommendations in the Guide for the Care and Use of Laboratory Animals of the University of Arizona (Protocol #: . All efforts were made to minimize animal suffering. All behavioral experiments were performed by experimenters who were blinded to the genotype, and sex, and treatment groups.
Sprague dawley rat pups
Manufactured by Inotiv
Sourced in United States
Sprague Dawley rat pups are a commonly used animal model in research. They are a breed of albino laboratory rats that are known for their docile temperament and robust health. The pups are often used in various studies, such as developmental, neurological, and behavioral research.
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Lab products found in correlation
3 protocols using sprague dawley rat pups
1
Sprague-Dawley Rat Electrophysiology Protocol
2
Postnatal Rat Developmental Study
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All experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Loma Linda University in accordance with the National Institute of Health (NIH) Guide for the Care and Use of Laboratory Animals. A total of 99 10‐day‐old unsexed Sprague Dawley rat pups (Envigo, Livermore, CA, USA) weighing 16‐20 g were used. Animals were housed in a controlled humidity and temperature room with a 12 hours light and dark cycle.
3
Skeletal Muscle Cell Proliferation
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Sprague-Dawley rat pups (1–2 days of age) were purchased from Envigo, Jerusalem, Israel. The pups were killed by decapitation and skeletal muscles were dissected from the thigh for subsequent preparation (see below). All experiments were conducted according to the guidelines of the National Institutes of Health for the care and use of laboratory animals and approved by the institutional review board of Bar-Ilan University (Ethical number 98-12-2014).
All chemicals were from Sigma-Aldrich unless stated otherwise. 3H-thymidine was from Perkin-Elmer (Waltham, MA, USA). Polyclonal antibody against an extracellular epitope of the inward rectifying K+ channel 2.1 (Kir2.1) was purchased from Alomone Labs (#APC-159, Jerusalem, Israel). Gambogic acid (GA) was purchased from Abcam (ab145183, Cambridge, UK). The potentiometric fluorescent dye, di-8-ANEPPS, was purchased from Biotium (#61014, Fremont, CA, USA).
All chemicals were from Sigma-Aldrich unless stated otherwise. 3H-thymidine was from Perkin-Elmer (Waltham, MA, USA). Polyclonal antibody against an extracellular epitope of the inward rectifying K+ channel 2.1 (Kir2.1) was purchased from Alomone Labs (#APC-159, Jerusalem, Israel). Gambogic acid (GA) was purchased from Abcam (ab145183, Cambridge, UK). The potentiometric fluorescent dye, di-8-ANEPPS, was purchased from Biotium (#61014, Fremont, CA, USA).
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