Drosophila melanogaster flies were raised on standard media; stocks were kept at room temperature and crosses were reared at 25°C or 29°C as indicated. Four Gal4 fly lines were used to induce transgene expression: dpp-Gal4, UAS-GFP/TM6B (Swarup et al., 2015 (link)), Dll-lacZ/Cyo; Hh-Gal4/TM6B (Hall et al., 2017 (link)), tj-Gal4/tj-Gal4 (a gift from Dr Nicholas Harden, Simon Fraser University; Vlachos et al., 2015 (link)), and apterous (ap)-Gal4 (Bloomington Drosophila Stock Center, 3041). Additional stocks used were: puc-LacZ (Martin-Blanco et al., 1998 (link)) and UAS-dsh (Bloomington Drosophila Stock Center, 9453). As controls, the Gal4 drivers were crossed with w1118 or UAS-GFP flies. To generate transgenic UAS stock lines, patient variant Flag-tagged DVL1 constructs were sent for integration into the attP40 locus on the second chromosome for generation of stably integrated fly strains (BestGene, CA, USA).
Tj gal4
Manufactured by Bloomington Drosophila Stock Center
Sourced in China
The Tj-Gal4 is a genetic tool used in Drosophila research. It serves as a driver line, expressing the yeast transcriptional activator Gal4 under the control of the tj (tailless) gene promoter. The Tj-Gal4 line can be used to drive the expression of target genes in a specific pattern during Drosophila development.
Automatically generated - may contain errors
Lab products found in correlation
Puc lacz, by Bloomington Drosophila Stock Center (1 mentions)
Dpp gal4, by Bloomington Drosophila Stock Center (1 mentions)
Uas hepca, by Bloomington Drosophila Stock Center (1 mentions)
Uas bskdn, by Bloomington Drosophila Stock Center (1 mentions)
Tub gal80ts, by Bloomington Drosophila Stock Center (1 mentions)
Uas dpprnai, by Bloomington Drosophila Stock Center (1 mentions)
Uas yfp rab5, by Bloomington Drosophila Stock Center (1 mentions)
Uas cdc42 rnai, by Bloomington Drosophila Stock Center (1 mentions)
Nos gal4, by Bloomington Drosophila Stock Center (1 mentions)
3 protocols using tj gal4
1
Drosophila Transgene Expression Protocols
2
Drosophila Genetic Toolkit for Rab5 Study
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Rab5Z1 was an EMS allele generated in our lab; Rab52 was from Marcos González; UAS-Rab5 GFP/TM3, UAS-Rab5 RNAi/CyO, and UAS-Rab5 RNAi/TM3 were from Xinhua Lin; C587-GAL4 was from Ting Xie; nosGAL4VP16 was from Ruth Lehmann; pucE69 (puc-lacZ) was from Joaquim Culi; kek1-lacZ is an enhancer trap line (BB142) from T. Schüpbach; UAS-YFP-Rab5 (BL24616), UAS-TSG101-RNAi (BL35710), UAS-Vps25-RNAi (BL26286), UAS-avl-RNAi (BL29546), UAS-Cdc42-RNAi (BL28021), dppP10638 (dpp-lacZ), tj-GAL4, tub-GAL80ts, UAS-dpp-RNAi (BL25782), UAS-bskDN, UAS-hepCA, and 2L deficiency flies were from Bloomington Drosophila Stock Center.
Fly stocks were maintained under standard culture conditions and all flies were dissected 0–2 days after eclosion unless otherwise indicated. For GAL4/GAL80ts controlled RNAi or gene expression, flies were raised at 18 or 25°, shifted to 29° upon eclosion, and aged at 29° for 5 days before dissection. Germline or somatic cyst cell specific clones were generated by expressing FLP with nosGAL4VP16 or C587GAL4 (Kawase et al. 2004 (link)), respectively.
Fly stocks were maintained under standard culture conditions and all flies were dissected 0–2 days after eclosion unless otherwise indicated. For GAL4/GAL80ts controlled RNAi or gene expression, flies were raised at 18 or 25°, shifted to 29° upon eclosion, and aged at 29° for 5 days before dissection. Germline or somatic cyst cell specific clones were generated by expressing FLP with nosGAL4VP16 or C587GAL4 (Kawase et al. 2004 (link)), respectively.
3
Drosophila Genetic Manipulation Protocols
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Flies were reared on standard cornmeal medium at 25°C. The following strains were used: w1118, daughterless-Gal4, nos-Gal4, tj-Gal4; Bloomington Drosophila Stock Center: UAS-Caf1-55-IR2 (catalog 34069), w[*];Caf1-55[p55-1]/TM3, Sb[1] (catalog 68168); UAS-Caf1-55 (provided by Rongwen Xi, National Institute of Biological Sciences, Beijing, China); and Tsinghua Fly Center: UAS-Caf1-55-IR1 (THU0589).
To generate UAS-RBBP7, UAS-RBBP7Δ, and UAS-Caf1-55Δ transgenic flies, we amplified the full-length RBBP7 cDNA, mutant RBBP7 cDNA, and mutant Caf1-55 cDNA and cloned each of them into separate pUAST-attb vectors. These constructs were then transformed into 25C6 line embryos using the standard P-element–mediated transgenesis protocol.
To generate UAS-RBBP7, UAS-RBBP7Δ, and UAS-Caf1-55Δ transgenic flies, we amplified the full-length RBBP7 cDNA, mutant RBBP7 cDNA, and mutant Caf1-55 cDNA and cloned each of them into separate pUAST-attb vectors. These constructs were then transformed into 25C6 line embryos using the standard P-element–mediated transgenesis protocol.
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